产品基本信息

产品名称：Screen Quest 活细胞cAMP检测

产品介绍

G蛋白偶联受体（GPCR）是药物发现计划中大的靶向受体之一。钙通量（通过Gq途径耦合）分析是筛选GPCR靶点的方法。然而，超过60%的已知GPCRs信号通过腺苷酸环化酶活性偶联到cAMP。现有的cAMP检测方法不仅需要细胞裂解，而且缺乏时间和空间分辨率。Screen Quest 活细胞cAMP检测以高通量的形式提供细胞内cAMP变化的实时监测，无需细胞裂解步骤。该方法通过含有外源性环核苷酸门控通道（CNGC）或混杂G蛋白Gα16的细胞系进行。该通道被细胞内cAMP水平升高，导致离子通量和细胞膜去极化，可通过荧光钙（如Calbryte 520 AM、Cal-520 AM、Fluo-8 AM或Fluo-4 AM和相应的免洗钙试剂盒）或荧光膜电位染料检测到。Gα16与特异性非Gq偶联受体的共表达将在受体刺激下产生细胞内钙信号。Screen Quest 活细胞cAMP检测提供细胞系和试剂，用于使用FLIPR、FDSS或其他等效的荧光酶标仪检测细胞内cAMP的变化。它已成功地用于测定Gs和Gi偶联GPCR活性。金畔生物是AAT Bioquest的中国代理商，为您提供优质的Screen Quest 活细胞cAMP检测。 

适用仪器

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图1. Screen Quest 活细胞cAMP测定原理

参考文献

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