产品基本信息

产品名称：氯离子荧光探针MQAE

CAS：162558-52-3

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：326.19

外观：黄色固体

溶剂：DMSO

激发波长（nm）：350

发射波长（nm）：460

产品介绍

氯离子荧光探针MQAE是美国AAT Bioquest生产的用于检测氯离子的荧光探针。氯化物指示剂，其荧光依赖于氯离子浓度。MQAE的荧光强度随着氯离子在细胞内的增加而成比例地减少。MQAE具有高水溶性和膜渗透能力，可以检测低于50 mM浓度的氯离子浓度而不受pH变化的影响。MQAE的检测灵敏度比SPQ更高，并且荧光效果也比SPQ要好一些，是一种目前最广泛应用的新型氯离子荧光探针。金畔生物是AAT Bioquest的中国代理商，为您提供优质的氯离子荧光探针MQAE。

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操作步骤

1. 用Krebs-HEPES缓冲液洗涤细胞3次。
2. 将5 mM的Cl探针溶液加入细胞液中，并在37℃下培养1小时。
3. 用Krebs-HEPES缓冲液洗涤细胞5次。
4. 使用带有图像分析系统的激光共聚焦显微镜检测细胞的荧光强度。

注1：MQAE的工作液可以配制于Krebs-HEPES缓冲液中；Krebs-HEPES缓冲液配方（20mM HEPES，128mM NaCl，2.5mM KCl，2.7mM CaCl₂，1mM MgCl₂，16mM glucose，pH7.4）

注2：MQAE标记细胞时的浓度通常为5-10mM。以约800-1000万cell/ml的细胞密度，在含有5-10mM MQAE的Krebs-HEPES缓冲液中孵育30-60分钟。通常一个探针的装载体系为100ul即可。

参考文献

Lotus Leaf Alkaloid Extract Displays Sedative–Hypnotic and Anxiolytic Effects through GABAA Receptor
Authors: Yan, Ming-Zhu and Chang, Qi and Zhong, Yu and Xiao, Bing-Xin and Feng, Li and Cao, Fang-Rui and Pan, Rei-Le and Zhang, Ze-Sheng and Liao, Yong-Hong and Liu, Xin-Min
Journal: Journal of agricultural and food chemistry (2015): 9277–9285

Chloride and bicarbonate have similar affinities to the intestinal anion exchanger DRA (down regulated in adenoma)
Authors: Lamprecht G, Schaefer J, Dietz K, Gregor M.
Journal: Pflugers Arch (2006): 307

On membrane motor activity and chloride flux in the outer hair cell: lessons learned from the environmental toxin tributyltin
Authors: Song L, Seeger A, Santos-Sacchi J.
Journal: Biophys J (2005): 2350

Reversal effect of haloperidol on doxorubicin resistance and chloride channel inhibition in erythroleukemic cell K562/Dox
Authors: Zhou JH, Wu DZ.
Journal: Zhonghua Zhong Liu Za Zhi (2005): 81

Cell volume response to hyposmotic shock and elevated cAMP in bovine trabecular meshwork cells 
Authors: Srinivas SP, Maertens C, Goon LH, Goon L, Satpathy M, Yue BY, Droogmans G, Nilius B.
Journal: Exp Eye Res (2004): 15

Measurement of halide efflux from cultured and primary airway epithelial cells using fluorescence indicators
Authors: Munkonge F, Alton EW, Andersson C, Davidson H, Dragomir A, Edelman A, Farley R, Hjelte L, McLachlan G, Stern M, Roomans GM.
Journal: J Cyst Fibros (2004): 171

Activation of CFTR by genistein in human airway epithelial cell lines
Authors: Andersson C, Servetnyk Z, Roomans GM.
Journal: Biochem Biophys Res Commun (2003): 518

Expression and regulation of the Na+-K+-2Cl- cotransporter NKCC1 in the normal and CFTR-deficient murine colon
Authors: Bachmann O, Wuchner K, Rossmann H, Leipziger J, Osikowska B, Colledge WH, Ratcliff R, Evans MJ, Gregor M, Seidler U.
Journal: J Physiol (2003): 525

Modulation of intracellular Cl- homeostasis by lectin-stimulation in Jurkat T lymphocytes
Authors: Lai ZF, Chen YZ, Nishi K.
Journal: Eur J Pharmacol (2003): 1

Coordinated regulation of gastric chloride secretion with both acid and alkali secretion
Authors: Coskun T, Baumgartner HK, Chu S, Montrose MH.
Journal: Am J Physiol Gastrointest Liver Physiol (2002): G1147