产品基本信息

产品名称：Gelite 橙色核酸凝胶染色试剂盒

储存条件：保存在冰箱-15℃避光干燥

保质期：12个月

产品物理化学光谱特性

外观：液体

激发波长（nm）：495

发射波长（nm）：540

样品实验方案

溶液配制

工作溶液

将1μLGelite 橙色（组分A）添加到200μL5X凝胶上样缓冲液（组分B）中。 通过用箔纸覆盖或将其置于黑暗中来保护工作溶液免受光照。

操作步骤

1.根据需要准备DNA样品。

2.将4 µL Gelite Orange工作溶液加入16 µL DNA样品中，并充分混合。 电泳前，在室温下孵育5-15分钟。

3.根据您的标准方案运行凝胶。

4.使用300 nm紫外线或254 nm透照器或使用长路径绿色滤光片（例如SYBR®滤光片或GelStar®滤光片）的基于激光的凝胶扫描仪对凝胶成像。

参考文献

A Broadly Reactive One-Step SYBR Green I Real-Time RT-PCR Assay for Rapid Detection of Murine Norovirus
Authors: Hanaki K, Ike F, Kajita A, Yasuno W, Yanagiba M, Goto M, Sakai K, Ami Y, Kyuwa S.
Journal: PLoS One (2014): e98108

A SYBR Green I based real time RT-PCR assay for specific detection and quantitation of Peste des petits ruminants virus
Authors: Abera T, Thangavelu A, Joy Chandran ND, Raja A.
Journal: BMC Vet Res (2014): 22

A SYBR-green I quantitative real-time reverse transcription-PCR assay for rabies viruses with different virulence
Authors: Wang L, Liu Y, Zhang S, Wang Y, Zhao J, Miao F, Hu R.
Journal: Virol Sin (2014): 131

Comparison of SYBR Green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes
Authors: Tajadini M, Panjehpour M, Javanmard SH.
Journal: Adv Biomed Res (2014): 85

Detection and characterization of Leishmania (Leishmania) and Leishmania (Viannia) by SYBR green-based real-time PCR and high resolution melt analysis targeting kinetoplast minicircle DNA
Authors: Ceccarelli M, Galluzzi L, Migliazzo A, Magnani M.
Journal: PLoS One (2014): e88845

Detection of Cardamom mosaic virus and Banana bract mosaic virus in cardamom using SYBR Green based reverse transcription-quantitative PCR
Authors: Siljo A, Bhat AI, Biju CN.
Journal: Virusdisease (2014): 137

Development and comparative evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of West Nile virus in human patients
Authors: Kumar JS, Saxena D, Parida M.
Journal: Mol Cell Probes. (2014)

Development and evaluation of SYBR Green-I based quantitative PCR assays for herpes simplex virus type 1 whole transcriptome analysis
Authors: Garvey CE, McGowin CL, Foster TP.
Journal: J Virol Methods (2014): 101

Development of a High-resolution Melting Analysis Method Based on SYBR Green-I for rs7216389 Locus Genotyping in Asthmatic Child Patients
Authors: Vali Z, Raz A, Bokharaei H, Nabavi M, Bemanian MH, Yazdi MS, Djadid ND.
Journal: Avicenna J Med Biotechnol (2014): 72

Development of a SYBR Green I based one-step real-time PCR assay for the detection of Hantaan virus
Authors: Jiang W, Wang PZ, Yu HT, Zhang Y, Zhao K, Du H, Bai XF.
Journal: J Virol Methods (2014): 145

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