产品基本信息

产品名称：ReadiUse DRAQ5染色液

CAS：252903-95-0

储存条件：-15℃避光干燥

保质期：12个月

产品物理化学光谱特性

分子量：485.41

溶剂：水

激发波长(nm)：646

发射波长(nm)：697

产品介绍

DRAQ5 是1,5-双{[2-（二甲基氨基）乙基]氨基} -4，8-二羟基蒽-9,10-二酮的商标名（DRAQ-5 是Biostatus Ltd.的商标） 。它是活细胞或固定细胞的远红色DNA染色剂，可以很容易地与其他荧光团（例如表达绿色荧光蛋白（GFP）融合蛋白的细胞）复用。它是亲脂性和膜渗透性染料，用于活细胞和固定细胞染色以及DNA含量定量。它也是与其他荧光报告基因一起用于荧光显微镜或高含量筛选（HCS）的理想选择。由于其远红外激发和发射，DRAQ-5染色剂可与许多其他荧光团多路复用。在涉及表达绿色荧光蛋白（GFP）融合蛋白的细胞的细胞增殖研究中，通常选择它来复核核并测量DNA含量。DRAQ-5与广泛的仪器平台上的许多操作方案。AAT Bioquest提供了此即用型实验方案，可帮助您进行荧光成像和流式细胞术测试。

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参考文献

DRAQ5 and Eosin (‘D&E’) as an Analog to Hematoxylin and Eosin for Rapid Fluorescence Histology of Fresh Tissues
Authors: K. N. Elfer
Journal: PLoS One (2016): e0165530

One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays
Authors: Sheng Dai, Rong Li, Yan Long, Steve Titus, Jinghua Zhao, Ruili Huang, Menghang Xia, Wei Zheng
Journal: Journal of Biomolecular Screening (2016): 1112–1124

Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production
Authors: Guoqiang Zhang, Yuping Lin, Xianni Qi, Lixian Wang, Peng He, Qinhong Wang, Yanhe Ma
Journal: Microbial cell factories (2015): 1

Short exposure to the DNA intercalator DRAQ5 dislocates the transcription machinery and induces cell death
Authors: E. Richard
Journal: Photochem Photobiol (2011): 256-61

Influence of the live cell DNA marker DRAQ5 on chromatin-associated processes
Authors: P. O. Mari
Journal: DNA Repair (Amst) (2010): 848-55

The use of DRAQ5 to monitor intracellular DNA in Escherichia coli by flow cytometry
Authors: F. Silva
Journal: J Fluoresc (2010): 907-14

DRAQ5-based, no-lyse, no-wash bone marrow aspirate evaluation by flow cytometry
Authors: R. W. Allan
Journal: Am J Clin Pathol (2008): 706-13

DRAQ5: improved flow cytometric DNA content analysis and minimal residual disease detection in childhood malignancies
Authors: K. Swerts
Journal: Clin Chim Acta (2007): 154-7

DRAQ5-based DNA content analysis of hematolymphoid cell subpopulations discriminated by surface antigens and light scatter properties
Authors: C. M. Yuan
Journal: Cytometry B Clin Cytom (2004): 47-52

A novel cell permeant and far red-fluorescing DNA probe, DRAQ5, for blood cell discrimination by flow cytometry
Authors: Smith PJ, Wiltshire M, Davies S, Patterson LH, Hoy T.
Journal: J Immunol Methods (1999): 131