产品基本信息

产品名称：DAPI（4′,6-二脒基-2-苯基吲哚二盐酸盐）

CAS：28718-90-3

储存条件：保存在冰箱-20℃干燥

保质期：6个月

产品物理化学光谱特性

分子量：350.25

溶剂：水

Ex(nm):358

Em(nm):461

产品介绍

DAPI 4,6-二脒基-2-苯基吲哚，二盐酸盐是美国AAT Bioquest生产的用于染色DNA的试剂，DAPI 是一种能与 DNA 紧密结合的荧光染料。 它广泛用于荧光显微镜。 由于 DAPI 可穿过完整的细胞膜，因此除固定细胞外，它还可用于活细胞染色。 对于荧光显微镜，DAPI 被紫外光激发。 当与双链 DNA 结合时，其最大吸收波长为 358 nm，最大发射波长为 461 nm。 DAPI 的一个缺点是它的排放相当广泛。 DAPI 也与 RNA 结合，尽管它不像与 DNA 结合那样发出强烈的荧光。 当与 RNA 结合时，它的发射波长会转移到 500 nm 左右。 DAPI 的蓝色发射便于多重分析，因为 DAPI 和绿色荧光分子（如荧光素和绿色荧光蛋白 (GFP)）或红色荧光染料（如德克萨斯红）之间的荧光重叠非常少。 除了标记细胞核外，DAPI 还用于检测细胞培养物中的支原体或病毒 DNA。金畔生物是AAT Bioquest的中国代理商，为您提供优质的DNA染色试剂/试剂盒。 

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参考文献

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Authors: T Nagatake, SI Hirata, T Koga, E Kuroda, S Kobari, H Suzuki, K Hosomi, N Matsumoto, Y Yanrismet, M Shimojou
Journal: Mucosal immunology (2019)

Low-Luminance Blue Light-Enhanced Phototoxicity in A2E-Laden RPE Cell Cultures and Rats
Authors: Cheng-Hui Lin, Man-Ru Wu, Wei-Jan Huang, Diana Shu-Lian Chow, George Hsiao, Yu-Wen Cheng
Journal: International journal of molecular sciences (2019): 1799

β-tricalcium phosphate composite ceramics with high compressive strength, enhanced osteogenesis and inhibited osteoclastic activities
Authors: Ye Tian, Teliang Lu, Fupo He, Yubin Xu, Haishan Shi, Xuetao Shi, Fei Zuo, Shanghua Wu, Jiandong Ye
Journal: Colloids and Surfaces B: Biointerfaces (2018)

δ-Tocopherol promotes genic gene expression via PGC-1α upregulation in 3T3-L1 cells
Authors: Rieko Tanaka-Yachi, Masato Shirasaki, Rena Otsu, Chie Takahashi-Muto, Hideki Inoue, Yoshinori Aoki, Taisuke Koike, Chikako Kiyose
Journal: Biochemical and biophysical research communications (2018)

A Novel Heterozygous Missense Mutation in GNAT1 Leads to Autosomal Dominant Riggs Type of Congenital Stationary Night Blindness
Authors: Christina Zeitz, Cécile Méjécase, Mathilde Stévenard, Christelle Michiels, Isabelle Audo, Michael F Marmor
Journal: BioMed Research International (2018)

Acrylamide-induced oxidative stress and inflammatory response are alleviated by N-acetylcysteine in PC12 cells: Involvement of the crosstalk between Nrf2 and NF-$kappa$B pathways regulated by MAPKs
Authors: Xiaoqi Pan, Xu Wu, Dandan Yan, Cheng Peng, Chaolong Rao, Hong Yan
Journal: Toxicology Letters (2018)

Augmenter of liver regeneration promotes mitochondrial biogenesis in renal ischemia–reperfusion injury
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Journal: Apoptosis (2018): 1–12

Glucocorticoid-activation system mediated glucocorticoid-insulin-like growth factor 1 (GC-IGF1) axis programming alteration of adrenal dysfunction induced by prenatal caffeine exposure
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Journal: Toxicology Letters (2018)

In vivo tailor-made protein corona of prodrug-based nanoassembly fabricated by redox dual-sensitive paclitaxel prodrug for the superselective treatment of breast cancer
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Journal: Biomaterials Science (2018)

Inactivation of the tight junction gene CLDN11 by aberrant hypermethylation modulates tubulins polymerization and promotes cell migration in nasopharyngeal carcinoma
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Journal: Journal of Experimental & Clinical Cancer Research (2018): 102

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