产品基本信息

产品名称：DAPI[4,6-二脒基-2-苯基吲哚，二盐酸盐]* 10 mM水溶液*

CAS：28718-90-3

储存条件：保存在冰箱-20℃干燥

保质期：6个月

产品物理化学光谱特性

分子量：350.25

溶剂：水

Ex(nm):358

Em(nm):461

产品介绍

DAPI 是一种能与 DNA 紧密结合的荧光染料。 它广泛用于荧光显微镜。 由于 DAPI 可穿过完整的细胞膜，因此除固定细胞外，它还可用于活细胞染色。 对于荧光显微镜，DAPI 被紫外光激发。 当与双链 DNA 结合时，其最大吸收波长为 358 nm，最大发射波长为 461 nm。 DAPI 的一个缺点是它的排放相当广泛。 DAPI 也与 RNA 结合，尽管它不像与 DNA 结合那样发出强烈的荧光。 当与 RNA 结合时，它的发射波长会转移到 500 nm 左右。 DAPI 的蓝色发射便于多重分析，因为 DAPI 和绿色荧光分子（如荧光素和绿色荧光蛋白 (GFP)）或红色荧光染料（如德克萨斯红）之间的荧光重叠非常少。 除了标记细胞核外，DAPI 还用于检测细胞培养物中的支原体或病毒 DNA。

点击查看光谱

参考文献

Astrocytic expression of the CXCL12 receptor, CXCR7/ACKR3 is a hallmark of the diseased, but not developing CNS
Authors: Malte Puchert, Fabian Pelkner, Gregor Stein, Doychin N Angelov, Johannes Boltze, Daniel-Christoph Wagner, Francesca Odoardi, Alexander Flügel, Wolfgang J Streit, Jürgen Engele
Journal: Molecular and Cellular Neuroscience (2017)

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Fuqiang Guo, Jiajia Shang, Hai Zhao, Kangrong Lai, Yang Li, Zhongxiong Fan, Zhenqing Hou, Guanghao Su
Journal: Colloids and Surfaces B: Biointerfaces (2017)

Legionella pneumophila infection activates bystander cells differentially by bacterial and host cell vesicles
Authors: Anna Lena Jung, Christina Elena Herkt, Christine Schulz, Kathrin Bolte, Kerstin Seidel, Nicoletta Scheller, Alexandra Sittka-Stark, Wilhelm Bertrams, Bernd Schmeck
Journal: Scientific Reports (2017)

Peptides mimicking viral proteins of porcine circovirus type 2 were profiled by the spectrum of mouse anti-PCV2 antibodies
Authors: Ling-Chu Hung, Cheng-Yao Yang, Ivan-Chen Cheng
Journal: BMC immunology (2017): 25

Poly (ADP-ribose) polymerase inhibitors activate the p53 signaling pathway in neural stem/progenitor cells
Authors: Akiko Okuda, Suguru Kurokawa, Masanori Takehashi, Aika Maeda, Katsuya Fukuda, Yukari Kubo, Hyuma Nogusa, Tomoka Takatani-Nakase, Shujiro Okuda, Kunihiro Ueda
Journal: BMC neuroscience (2017): 14

Apoptotic effect of the selective PPARβ/δ agonist GW501516 in invasive bladder cancer cells
Authors: Adeline Péchery, Sylvie Fauconnet, Hugues Bittard, Isabelle Lascombe
Journal: Tumor Biology (2016): 1–14

Carbon nanotube–Protamine hybrid: Evaluation of DNA cell penetration
Authors: Cécile Caoduro, Raoudha Kacem, Khaoula Boukari, Fabien Picaud, Claire-Hélène Brachais, David Monchaud, Christophe Borg, Hatem Boulahdour, Tijani Gharbi, Régis Delage-Mourroux
Journal: Carbon (2016): 742–752

Identification of epigenetic factors regulating the mesenchyme to epithelium transition by RNA interference screening in breast cancer cells
Authors: Jean-Marc Gregoire, Laurence Fleury, Clara Salazar-Cardozo, Frédéric Alby, Véronique Masson, Paola Barbara Arimondo, Frédéric Ausseil
Journal: BMC cancer (2016): 700

Legionella pneumophila-Derived Outer Membrane Vesicles Promote Bacterial Replication in Macrophages
Authors: Anna Lena Jung, Cornelia Stoiber, Christina E Herkt, Christine Schulz, Wilhelm Bertrams, Bernd Schmeck
Journal: PLoS Pathog (2016): e1005592

Redox-Sensitive Citronellol–Cabazitaxel Conjugate: Maintained in Vitro Cytotoxicity and Self-Assembled as Multifunctional Nanomedicine
Authors: Peng Xue, Dan Liu, Jing Wang, Na Zhang, Jiahua Zhou, Lin Li, Weiling Guo, Mengchi Sun, Xiangfei Han, Yongjun Wang
Journal: Bioconjugate chemistry (2016): 1360–1372

相关产品