产品基本信息

产品名称：吖啶橙

CAS：65-61-2

储存条件：保存在冰箱-20℃干燥

产品物理化学光谱特性

分子量：301.82

外观：亮黄色

溶剂：DMSO

激发波长(nm)：500

发射波长(nm)：526

产品介绍

吖啶橙是一种核酸选择性荧光阳离子染料，可用于细胞周期测定。 它是细胞可渗透的，并通过插入或静电吸引力与DNA和RNA相互作用。当与DNA结合时，它与荧光素在光谱上非常相似，激发大值为502nm，发射大值为525nm（绿色）。当它与RNA结合时，激发大值移至460nm（蓝色），发射大值移至650nm（红色）。染料通常用于落射荧光显微镜。

点击查看光谱

染色细胞示例

以下程序适用于大多数细胞类型。生长培养基，细胞密度，其他细胞类型的存在和其他因素可能影响染色。玻璃器皿上的残留洗涤剂也可能影响许多生物的真实或明显染色，导致在有或没有细胞存在的溶液中出现明亮染色的物质。

1）制备1-10mM DMSO储备溶液。如果储存在-20°C，DMSO储备液可以保存6个月。

2）使用适合您样品的固定方案。

3）通过离心沉淀细胞，并将细胞重悬于缓冲盐溶液或培养基中，在pH 7.4下进行佳染料染色。培养物中的贴壁细胞可以在盖玻片上或细胞培养孔中原位染色。

4）使用1和10μM之间的浓度添加AO染色并孵育15至60分钟作为指导。

注意：在初的实验中，好在整个建议的范围内尝试几种染料浓度，以确定产生佳染色的浓度。

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参考文献

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