产品基本信息

产品名称：Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM *

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：905.79

溶剂：水

激发波长(nm)：N/A

发射波长(nm)：N/A

产品介绍

Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM *是美国AAT Bioquest生产的荧光染料，生物素修饰的dCTP类似物广泛用于各种非放射性DNA标记反应，包括切口平移，随机引物标记，cDNA标记和3′-末端标记。已经显示生物素化的探针以与非生物素化的探针相同的速率和相同的程度与同源核酸杂交。亲和素和链霉亲和素可以检测到杂交的生物素化DNA探针。将生物素-14-dCTP酶促地掺入DNA / cDNA中，以替代dCTP。随后使用与辣根过氧化物酶（HRP），碱性磷酸酶（AP），荧光染料或琼脂糖/磁珠偶联的抗生蛋白链菌素检测所得的生物素标记的DNA / cDNA探针。在胞嘧啶N4位置和生物素标签之间的14原子间隔子将CTP碱基与生物素标签分开，从而确保了尼克翻译的佳性能。对于使用Taq聚合酶进行PCR掺入实验，可以将50％Biotin-14-dCTP / 50％dCTP比率用于Nick翻译。或者，您可以在测试条件下确定佳比率。金畔生物是AAT Bioquest的中国代理商，为您提供优质的Tris缓冲液（pH 7.5）中的生物素-14-dCTP * 1 mM *。

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