D-荧光素乙酯 CAS 135251-85-3-AAT Bioquest荧光染料

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D-荧光素乙酯 CAS 135251-85-3价格 2823
产品规格

5 mg

产品货号

D-荧光素乙酯 CAS 135251-85-3

产品参数
Ex (nm) 385 Em (nm) 529
分子量 308.38 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:D-荧光素乙酯

CAS:135251-85-3

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:308.38

溶剂:DMSO

激发波长(nm):328

发射波长(nm):533

 

产品介绍

D-荧光素乙酯是美国AAT Bioquest生产的荧光染料,D-荧光素乙酯含有在羧基位置连接的甲基部分,因此防止它被萤火虫荧光素酶识别。 然而,酯通过脂肪酶活性有效地水解,并且所得的D-荧光素被荧光素酶很好地识别。 D-荧光素甲酯是用于在均相测定中或在与荧光素酶及其辅因子组合的细胞裂解物样品中化学发光测量脂肪酶活性的敏感底物。金畔生物是AAT Bioquest的中国代理商,为您提供优质的D-荧光素乙酯。 

Luc的Ex/Em及其他相关内容链接:Spectral properties of luciferin and EGFP. A: Normalized excitation and… | Download Scientific Diagram (researchgate.net)

金畔问号:荧光素酶到底该如何检测?

 

试剂应用文献

Genome-wide microRNA analysis identifies miR-188-3p as novel prognostic marker and molecular factor involved in colorectal carcinogenesis
Authors: Pichler, Martin and Stiegelbauer, Verena and Vychytilova-Faltejskova, Petra and Ivan, Cristina and Ling, Hui and Winter, Elke and Zhang, Xinna and Goblirsch, Matthew and Wulf-Goldenberg, Annika and Ohtsuka, Masahisa and others, undefined
Journal: American Association for Cancer Research (2016): clincanres–0497
 
C3-Luc Cells Are an Excellent Model for Evaluation of Cellular Immunity following HPV16L1 Vaccination
Authors: Li, Li-Li and Wang, He-Rong and Zhou, Zhi-Yi and Luo, Jing and Wang, Xiao-Li and Xiao, Xiang-Qian and Zhou, Yu-Bai and Zeng, Yi
Journal: PloS one (2016): e0149748
 
Identification of a novel protein kinase a inhibitor by bioluminescence-based screening
Authors: Ishimoto, Tetsuya and Azechi, Kenji and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2015): 1969–1974
 
Identification of a Novel Protein Kinase A Inhibitor by Bioluminescence-Based Screening
Authors: Ishimoto, Tetsuya and Azechi, Kenji and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2015): 1969–1974
 
Discovery of novel adenylyl cyclase inhibitor by cell-based screening
Authors: Mano, Hiroki and Ishimoto, Tetsuya and Okada, Takuya and Toyooka, Naoki and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2014): 1689–1693
 

参考文献

Mutagenesis of solvent-exposed amino acids in Photinus pyralis luciferase improves stability and pH-tolerance
Authors: Law GH, G and elman OA, Tisi LC, Lowe CR, Murray JA.
Journal: Biochem J (2006): 305
 
Determination of p-glycoprotein ATPase activity using luciferase
Authors: Matsunaga T, Kose E, Yasuda S, Ise H, Ikeda U, Ohmori S.
Journal: Biol Pharm Bull (2006): 560
 
Expression of firefly luciferase in Candida albicans and its use in the selection of stable transformants
Authors: Doyle TC, Nawotka KA, Purchio AF, Akin AR, Francis KP, Contag PR.
Journal: Microb Pathog (2006): 69
 
PET imaging and optical imaging with D-luciferin [11C]methyl ester and D-luciferin [11C]methyl ether of luciferase gene expression in tumor xenografts of living mice
Authors: Wang JQ, Pollok KE, Cai S, Stantz KM, Hutchins GD, Zheng QH.
Journal: Bioorg Med Chem Lett (2006): 331
 
Bioluminescence of monolayers of firefly luciferase immobilized on graphite
Authors: Palomba S, Berovic N, Palmer RE.Journal: Langmuir (2006): 5451
 
Bovine serum albumin displays luciferase-like activity in presence of luciferyl adenylate: insights on the origin of protoluciferase activity and bioluminescence colours
Authors: Viviani VR, Ohmiya Y.
Journal: Luminescence (2006): 262
 
Noninvasive bioluminescence imaging of luciferase expressing intracranial U87 xenografts: correlation with magnetic resonance imaging determined tumor volume and longitudinal use in assessing tumor growth and antiangiogenic treatment effect
Authors: Szentirmai O, Baker CH, Lin N, Szucs S, Takahashi M, Kiryu S, Kung AL, Mulligan RC, Carter BS.
Journal: Neurosurgery (2006): 365
 
Two different domains of the luciferase gene in the heterotrophic dinoflagellate Noctiluca scintillans occur as two separate genes in photosynthetic species
Authors: Liu L, Hastings JW.
Journal: Proc Natl Acad Sci U S A. (2006)
 
Effect of different salts and detergents on luciferin-luciferase luminescence of the enchytraeid Fridericia heliota
Authors: Rodionova NS, Petushkov VN.
Journal: J Photochem Photobiol B (2006): 123
 
Evaluation of firefly luciferase bioluminescence mediated photodynamic toxicity in cancer cells
Authors: Schipper ML, Patel MR, Gambhir SS.
Journal: Mol Imaging Biol (2006): 218

 

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