即用型ABTS溶液 适合HRP标记的ELISA检测 CAS 30931-67-0-AAT Bioquest荧光染料

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即用型ABTS溶液 适合HRP标记的ELISA检测 CAS 30931-67-0价格 2517
产品规格

1 L

产品货号

即用型ABTS溶液 适合HRP标记的ELISA检测 CAS 30931-67-0

产品参数
Ex (nm) Em (nm)
分子量 溶剂
存储条件 在2-8度冷藏保存, 避免光照
产品概述

即用型ABTS溶液是美国AAT Bioquest生产的ELISA检测试剂,辣根过氧化物酶(HRP)和HRP缀合物在过氧化氢存在下促进ABTS氧化,将ABTS转化为其蓝绿色氧化产物。该显色反应广泛用于ELISA测定中的HRP定量。氧化的ABTS产物具有420nm的大吸收,可以用分光光度计容易地跟踪。ReadiUse™ABTS解决方案针对在微孔板或试管中使用HRP或HRP标记的缀合物和过氧化氢的ELISA测定进行了优化。我们的ABTS解决方案可以在一次添加中完成HRP反应。在过氧化氢存在下,当与HRP或HRP缀合物反应时,测定溶液将其颜色改变为浅绿色。金畔生物是AAT Bioquest的中国代理商,为您提供优质的即用型ABTS溶液。 

 

适用仪器


光吸收酶标仪  
吸收: 420nm
推荐孔板: 透明底板
实验方案

样品实验方案

1.在HRP标记的试剂温育后洗涤测定板。

2.每孔加入100μLReadiUse ABTS溶液。

3.将板在室温下孵育15-30分钟。注意:孵育时间取决于测定条件。

4.用ELISA酶标仪测量415±10 nm(大420 nm)的吸光度信号。注意:如果需要,可以通过向0.1M柠檬酸中加入等体积的1%SDS或0.01%叠氮化钠来终止反应。
 
 
参考文献

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Journal: Chemistry (2009): 1107

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Journal: Chemistry (2009): 3411

Degradation of glyphosate and other pesticides by ligninolytic enzymes
Authors: Pizzul L, Castillo MD, Stenstrom J.
Journal: Biodegradation. (2009)

Plant extracts with anti-inflammatory properties–a new approach for characterization of their bioactive compounds and establishment of structure-antioxidant activity relationships
Authors: Amaral S, Mira L, Nogueira JM, da Silva AP, Helena Florencio M.
Journal: Bioorg Med Chem (2009): 1876

Self-assembly of enzymes on DNA scaffolds: en route to biocatalytic cascades and the synthesis of metallic nanowires
Authors: Wilner OI, Shimron S, Weizmann Y, Wang ZG, Willner I.
Journal: Nano Lett (2009): 2040

Supramolecular cocaine-aptamer complexes activate biocatalytic cascades
Authors: Freeman R, Sharon E, Tel-Vered R, Willner I.
Journal: J Am Chem Soc (2009): 5028

Effects of mutations in the helix G region of horseradish peroxidase
Authors: Ryan BJ, O’Fagain C.
Journal: Biochimie (2008): 1414

Full-field photonic biosensors based on tunable bio-doped sol-gel glasses
Authors: Llobera A, Cadarso VJ, Darder M, Dominguez C, Fernandez-Sanchez C.
Journal: Lab Chip (2008): 1185

Simultaneous total antioxidant capacity assay of lipophilic and hydrophilic antioxidants in the same acetone-water solution containing 2% methyl-beta-cyclodextrin using the cupric reducing antioxidant capacity (CUPRAC) method
Authors: Ozyurek M, Bektasoglu B, Guclu K, Gungor N, Apak R.
Journal: Anal Chim Acta (2008): 28

Broccoli processing wastes as a source of peroxidase
Authors: Duarte-Vazquez MA, Garcia-Padilla S, Garcia-Almendarez BE, Whitaker JR, Regalado C.
Journal: J Agric Food Chem (2007): 10396

 

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