由于环境因素和细胞内的正常代谢过程造成的DNA损伤，每个细胞每天都会发生1,000到1,000,000个。虽然这些只占人类基因组约60亿个碱基中的一小部分，但如果关键基因损伤未及时修复，可能会阻碍细胞的正常生理功能，进而增加癌变可能。彗星实验，或称单细胞凝胶电泳（Single cell gel electrophoresis，SCGE），是一种测量单个细胞DNA损伤的常用技术。其原理很简单，即在电泳场中，将受损细胞DNA（包含片段和链断裂）与完整的DNA分离，通过显微镜可观察到损伤细胞呈现出典型的彗星状尾巴，然后通过测量计算彗尾大小对比出细胞DNA损伤的程度。因为彗星实验的特点，该方法几乎被用来评估任何类型的真核细胞的 DNA 修复能力，包括双、单链断裂的不同的 DNA 损伤情况。是一种能快速、大通量检测真核细胞DNA损伤进而判别遗传毒性的技术。
彗星实验结果图
彗星实验原理虽简单，但操作繁琐，需要丰富的实验经验和技巧，尤其常常出现的“脱胶”问题，困扰了许多科研人员。除此之外，有时为了跑出完美的“彗星”图案放在paper里，还需要重复做许多次实验，费时费力。为了解决上述问题，我们推荐CellBiolabs的OxiSelectTMComet Assay Kit即彗星实验试剂盒来检测细胞的DNA损伤。该试剂盒不仅能让彗星实验化繁为简，还有两种不同规格（3孔和96孔）的细胞电泳凝胶板供选择，让少量样本和大量样本的DNA损伤检测通通轻松hold住。用该试剂盒做彗星实验流程如下图。
除了操作简便，OxiSelectTMComet Assay Kit还有以下优点：
1) 适用于各种DNA损伤检测，是一款非常好用的DNA损伤检测筛选工具；
2) 试剂盒中的载玻片经过特殊处理以粘附低熔点琼脂糖，避免“脱胶”问题出现；
3) 采用特殊的DNA荧光染料，能有效降低背景干扰，更加方便读取实验结果。
彗星实验试剂盒信息：
|
品名 |
货号 |
规格 |
说明 |
|
OxiSelectTM Comet Assay Kit (3-Well Slides) |
STA-350 |
15 assays |
试剂盒内有5张3孔载玻片和彗星实验所需的低熔点琼脂糖、裂解液及DNA荧光染料等，共可检测15个样品。 |
|
OxiSelectTM Comet Assay Kit (3-Well Slides) |
STA-351 |
75 assays |
试剂盒内有25张3孔载玻片和彗星实验所需的低熔点琼脂糖、裂解液及DNA荧光染料等，共可检测75个样品。 |
|
OxiSelectTM Comet Assay Kit (96-Well Slides) |
STA-355 |
96 assays |
试剂盒内有1张96孔载玻片和彗星实验所需的低熔点琼脂糖、裂解液及DNA荧光染料等，共可检测96个样品。 |
为了满足客户更多样的实验需求，彗星实验试剂盒内特殊处理电泳载玻片还可以单独购买，详情如下：
|
品名 |
货号 |
规格 |
产品图片 |
|
Comet Assay Slides, 3-Well |
STA-352 |
5 slides |
|
|
STA-353 |
25 slides |
||
|
Comet Assay Slides, 96-Well |
STA-356 |
1 slides |
|
|
STA-356-5 |
5 slides |
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