关东化学株式会社官网 关东化学试剂代理商
日本关东化学官网 日本关东化学中国代理
日本关东化学株式会社官网 关东化学试剂官方代理商
日本关东化学试剂授权代理商
关东化学株式会社是日本著名的综合试剂厂家(http://www.chemdrug.com/company/) 它的高质量产品(http://www.chemdrug.com/invest/)在国际上享有极高的声誉。不仅在中国国内 多年来在日本国内、亚洲地区、美国、欧洲等地都深受厚爱。为了保证所有试剂产品的质量 关东化学株式会社的全工厂都取得了ISO 9001和ISO/IEC 17025质量管理体系认证 建立了严格的品质管理体系。1998年 全工厂又在日本试剂行业中率先取得了ISO 14001环境管理体系认证。关东化学株式会社不仅是一家拥有先端技术的生产企业(http://www.chemdrug.com/company/) 也是一家重视环境安全以及人类社会健康(http://www.chemdrug.com/article/7/)和谐的可信赖企业。
无论是一般试剂还是特殊试剂、从零售到大量批发 我公司都会竭尽全力地为您提供优质服务、满足您的需求。
ACROS OrganicsACROS ORGANICS是全世界享有盛誉的精细化学品供应商，是有机化学和精细化学产品行业的领导者。ACROS ORGANICS凭借不断发展创新的产品和服务以满足有机、医药、分析和生化领域的各类研发和生产的产品需求。
美国Wheaton Science 美国WHEATON公司是世界著名的包装材料制造厂商之一。其产品涉及医药，化妆品，食品等各个领域，主要包括西林瓶，模制瓶等。每种产品都设计合理，制作精良，有其独到之处。
美国wheaton W219929-PGL45粉色盖试剂瓶
世尔科技（纽约证交所代码：TMO）是科学服务领域的世界领导者。公司年销售额超过200亿美元，在全球拥有约65,000名员工。
我们的使命是携手客户，让世界更健康、更清洁、更安全。我们帮助客户加速生命科学领域的研究、解决在分析领域所遇到的复杂问题与挑战，促进医疗诊断发展、加速药物上市进程、提高实验室生产力。
借助于首要品牌 Scientific、Applied Biosystems、Invitrogen、Fisher Scientific和Unity Lab Services，我们领先推出结合创新技术、便捷采购和全方位服务的整体解决方案。欲了解更多信息，请浏览公司网站
JinPanBio (上海金畔生物科技有限公司) Room 103, Building 32, No.669, Dongjing Rord, Pudong New Area, Shanghai, China Tel.: +86-21-50837765 Mobile: 86-18301939375 email: info@jinpanbio.com 上海金畔生物官网：http://www.jinpanbio.cn 上海金畔生物Lumiprobe官网：http://lumiprobe.jinpanbio.com/ Sigma代理 Abcam代理 CST代理 Santa Cruz代理 Biolegend代理 ebioscience代理 Invitrogen代理 millipore代理 BD流式抗体代理 GeneTe x抗体代理 Novus抗体代理 R&D代理 Biovison代理 Jackson代理 MBL抗体代理 ProSpec抗体代理 Bethyl抗体代理 Antibody Revolution抗体代理 Torrey Pines Biolabs代理 Amresco代理 MPbio代理 Laysan bio代理 NANOCS代理 Avanti代理 wako代理 lumiprobe代理（活性荧光染料） NEB酶代理 Roche酶代理 Toyobo酶代理 USP代理 EP代理 Dr代理 TRC代理 TCI代理 Reagecon代理 Megazyme代理 Hampton代理（蛋白结晶） whatman代理（滤膜滤纸） GE代理（蛋白纯化） Corning康宁代理 Axygen代理 Falcon代理 NISSUI日水代理 Himedia代理 OXOID代理 BD培养基代理 Ludger代理（糖蛋白分析产品） Eppendorf代理 Labnet代理 标准品代理 抗体代理 酶试剂代理 培养基代理 耗材代理 Elisa试剂盒代理 。
日本日油NOF 药用的修饰性PEG代理商
NOF produces the world’s best quality Activated PEGs from our high-quality methoxypoly(ethylene glycol) (mPEG). NOF also supplies our proprietary high-purity branched, multi-arm and hetero-functional Activated PEGs tailored to meet our customers’ PEG Drugs. NOF can supply Activated PEGs produced under GMP standards in our state-of-the-art GMP facility. NOF can help you to modify your drugs by using our PEGylation technology and our high-quality Activated PEGs.
Nof从我们的高质量甲氧基聚乙二醇(MPEG)中生产出世界上优质的活化支架.NOF还提供我们的专利高纯度分支，多臂和异功能活化钉定制，以满足我们的客户的PEG药物。在我们先进的gmp设施中，Nof可以提供按GMP标准生产的活化钉.Nof可以通过使用我们的PEG化技术和我们的高质量的激活钉来帮助您修改您的药物。
Activated PEGs
Overview
PEGylation
Functional Group
Quality
GMP Supply
Custom Mfg
Overview
NOF has been producing a high-quality activated PEGs, SUNBRIGHT series, which possess the most suitable quality for preparation of physiologically active drug and biologics. SUNBRIGHT series are characterized as extremely high-purity PEGs with a variety of functional groups, whereby the products of this series are recognized as optimal PEGylation drugs. As illustrated in this figure, application of the activated PEGs with various functional groups enable introduction of the PEG chains into drugs, enzymes, phospholipids and other biologics. Covalent conjugation of hydrophobic macromolecules with activated PEGs leads to the formation of macromolecular micelles (Polymer Micelles), which allow homogeneous dispersion of hydrophobic drugs in aqueous media. Furre, when the PEGylated phospholipids are used as liposomes, the aqueous layer can be added to its surface, with homogeneous dispersal of the liposome preparations encapsulating drugs within them.
As stated above, PEGylation plays an important role in the stabilization of drugs, reduction of their antigenicity and decrease in the drug doses, besides augmenting the targeting ability via binding biologics onto their surfaces. We can do supply our activated PEGs from small scale reagent grade to huge amount GMP grade with high purity.
PEGylation
PEGylation technology is applied for biologics modifications such as cytokines, antibody fragment, interleukins, hormones, oligonucleotides and some of proteins and peptides.
PEGylation can be effected to
Improving Bioavailability
Prolonging Blood Circulation
Maximizing Pharmacokinetics
Low-profiling Immunogenicity
NOF provides high-performance activated PEGs for PEGylated drugs from early development stage to commercial use with our 20 years of experience of manufacturing high-quality methoxy polyethylene glycol, which is a starting material of activated PEG for pharmaceutical area. Our activated PEGs have a narrow polydisperity and low diol content with wide range of molecular weight, from 2kDa to 80kDa.
PEG -conjugation
Analysis of PEG-ALD-Insulin by SDS-PAGE with Coomassie brilliant blue. Insulin was reacted with NOF PEG-ALD at 4°C. The PEGylated insulin was purified on Q Sepharose Fast Flowcolumn.
lane M molecular weight markers
lane 1 Insulin
lane 2 PEG(ME-050AL)-insulin eluate
lane 3 PEG(ME-100AL)-insulin eluate
lane 4 PEG(ME-200AL)-insulin eluate
lane 5 PEG(ME-300AL)-insulin eluate
Coupling of PEG derivatives to Proteins: examples of conditions
Coupling of PEG-NHS derivatives to protein amines (PEG-NHS + Protein-NH 2) Conditions 1: 50 mM phosphate buffer (pH 7.2), 4℃, 6 hrs *1)
Conditions 2: Borate-phosphate buffer (pH 8.0), 25℃, 2 hrs *2)
Coupling of PEG-Aldehyde derivatives to the NH 2 group of proteins (PEG-Ald + Protein-NH 2)
Conditions : sodium cyanoborohydride (10 eq . .), 4 deg C, 20 hrs *3)
Coupling of PEG-Maleimide derivatives to the SH group of proteins (PEG-Maleimide + Protein-SH)
Conditions: 100 mM phosphate buffer (pH 6.5), 4 deg C, 4 hrs
Coupling of PEG-NH 2 derivatives to the COOH group of proteins (PEG-NH 2 + Protein-COOH)
Conditions: 50mM phosphate buffer (pH 7.2), WSC (2 eq.), 4 deg C, 10 hrs
Coupling of PEG-p-Nitrophenyloxycarbonyl derivatives to the NH 2 group of proteins (PEG-NP + Protein-NH 2)
Conditions: borate-phosphate buffer (pH 8.0-8.3), r.t, overnight *2)
References:
*1) Abuchowski, A., et al. (1984) Cancer Biochem. Biophys. 7, 175.
*2) Sartore, L., et al. (1991) Appl. Biochem. Biotechnol. 27, 45
*3) US 5,824,784
Functional Group
We supply different series of PEG derivatives with various versatile functional groups.
Quality
Purity of NOF’s activated products
We use our advanced technologies to measure the quality of our activated PEGs. The following are the typical analysis data for each type of activated PEGs which are measured by NMR or HPLC methods. Please note that those numbers are not specification of the product.
NHS type
CS type mPEG-succinimidyl succinate (Mw 2k and 5k) : activity over 95% (NMR)
GS type mPEG-succinimidyl glutarate (Mw 5k, 10k, 20k, 30k and 40k) : activity around 95% (NMR)
AS type mPEG-succinimidyl methyl ester (Mw 2k and 5k) : activity around 95% (NMR)
HS type mPEG-succinimidyl pentyl ester(Mw 5k, 10k, 20k, 30k and 40k): activity around 95% (NMR)
TS type mPEG-succinimidyl carbonate(Mw 5k, 10k, 12k, 20k, 30k and 40k): activity over 95% (HPLC)
pNP type (NP series)
mPEG-p-nitrophenyl (Mw 2k, 5k, 10k, 20k, 30k and 40k): activity over 95% (HPLC)
Aldehyde type(AL series)
mPEG-propylaldehyde (Mw 5k, 10k, 20k, 30k and 40k): activity over 85% (HPLC). The activity for Mw20k, it is over 92% as actual data by HPLC.
Amine type
PA type mPEG-propylamine (Mw 2k, 5k, 10k, 12k, 20k, 30k and 40k): activity over 95% (HPLC)
EA type mPEG-ethylamine (Mw 5k, 10k, 20k, 30k and 40k): activity over 95% (HPLC)
thiol type(SH series)
mPEG-thiol (Mw 2k, 5k, 10k, 20k, 30k and 40k): activity around 95% (HPLC)
Maleimide type(MA series)
mPEG-maleimide (Mw 2k, 5k, 10k, 20k, 30k and 40k): activity around 95% (HPLC)
Oxyamine type(CA series)
mPEG-oxyamine (Mw 10k, 20k, 30k and 40k): activity around 95% (HPLC)
Iodoacetoamide type(IA series)
mPEG-irdoacetoamide (Mw 20k, 30k and 40k): activity around 90% (HPLC)
Analytical Technology
NOF has more than 20 years history for manufacturing of high-quality mPEGs and Activated PEGs. These experiences enable NOF to have thorough knowledge of PEGs and considerable achievement in analysis of Activated PEGs. We also have invented innovative analytical methods for assaying purity of activated PEGs and for determining impurity levels. (*Patent No.: JP4543917) Previously, the activity for activated PEGs is measured by NMR. However, this method is not very good detection due to polymer, especially for high molecular weight. We developed the analysis method by using HPLC. As the result of the analysis, the activity sometime seems lower than the result of NMR because main peak can completely separate from non-active peak and activated impurity peaks (like dimer).
HPLC analysis for M-PEG-COOH
GPC Analysis of M-PEG5K and M-PEG-30K
MALDI-TOF-MS Analysis of M-PEG5K and M-PEG-30K
GMP Supply
cGMP Manufacturing Facilities
NOF Activated PEGs, SUNBRIGHT® series are produced in facilities with state-of-the-art technology, operated under cGMP. The cGMP facilities are capable of producing small to large batch sizes, using proprietary know-how on scale-up procedure, depending on customers’ needs. These cGMP facilities have been audited by pharmaceutical companies throughout the world. NOF receives high reputation from our customers.
Research & Development
Our R&D facility was opened in December 2005. This allows us to continue our development of novel activated PEGs and technologies for PEGylation.
Custom Mfg
Research & DevelopmentOur R&D facility was opened in December 2005. This allows us to continue our development of novel activated PEGs and technologies for PEGylation.
Custom Manufacturing
NOF has over 20 years of experience in supplying high-quality methoxy polyethylene glycol (mPEG) for pharmaceutical market. The mPEG is starting material of PEG derivatives, which are patented through our proprietary technology. Based on extensive range of technological know-how and capabilities, NOF offers custom manufacturing of Activated PEGs from early stage development to commercial scale. Sizewise, we can offer gram size synthesis to kg size for non GMP product. We also offer cGMP custom synsthesis.
Please feel free to contact us : Contact form
1. Mono-functional Linear PEGs
2. Bi-Functional PEGs
3. Multi-Arm PEGs
4. Branched PEGs
5. Forked PEGs
6. Heterofunctional PEGs
7. Single Molecule PEGs PUREBRIGHT® Series
8. Poly-Hydroxyl Activated PEGS
10. Biodegradable Polymers for gene / nucleic acid Delivery SUNBRIGHT® ME-DT & DT series
11. Biodegradable block polymer for drug delivery
12.PEGylation Service
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PUREBRIGHT® 2TS-B12-AZ
Molecular weight: 1555.66
PUREBRIGHT® 2TS-B12-BO
Molecular weight: 1530.65
PUREBRIGHT® 2TS-B12-DB
Molecular weight: 1717.85
PUREBRIGHT® 2TS-B12-MA
Molecular weight: 1581.65
PUREBRIGHT® A-L08-PC
X = 8 Molecular weight: 441.51
PUREBRIGHT® A-L12-PC
X=12 Molecular weight: 617.72
PUREBRIGHT® BO-L08-PC
X=8 Molecular weight: 541.63
PUREBRIGHT® BO-L12-PC
X=8 Molecular weight: 717.84
PUREBRIGHT® MA-L08-PC
X=8 Molecular weight: 592.63
PUREBRIGHT® MA-L08-PS
X=8 Molecular weight: 689.71
PUREBRIGHT® MA-L12-PC
X=12 Molecular weight: 768.84
PUREBRIGHT® MA-L12-PS
X=12 Molecular weight: 865.92
