上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
Screen Quest 10X细胞染色缓冲液 *含酚红*价格 1386
产品规格
产品货号
| Ex (nm) | – | Em (nm) | – |
| 分子量 | N/A | 溶剂 | – |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品基本信息
产品名称：Screen Quest 10X细胞染色缓冲液
储存条件：-15℃避光防潮
保质期：24个月
产品介绍
即用型缓冲液，针对荧光细胞成像进行了优化。 在某些情况下，此缓冲液可明显增强成像信号。金畔生物是AAT Bioquest的中国代理商，为您提供优质的Screen Quest 10X细胞染色缓冲液。
图示
 图1.带有Phenol Red Plus 的Screen Quest 10X细胞染色缓冲液的图 |
 图2.使用Cal-520 AM在CHO-M1细胞中测量了ATP剂量反应。 将CHO-M1细胞以50,000个细胞/ 100 µL /孔在黑色96孔板中过夜接种。 加入含1X Phenol Red Plus 细胞染色缓冲液的HH缓冲液中的100 µL 10ug / ml Cal-520 AM，并在37oC下孵育60分钟。 加入ATP（50µL /孔）以达到指示的浓度。 |
参考文献
Kinetic characterization of novel NR2B antagonists using fluorescence detection of calcium flux
Authors: Bednar B, Cunningham ME, Kiss L, Cheng G, McCauley JA, Liverton NJ, Koblan KS.
Journal: J Neurosci Methods (2004): 247
Novel fluo-4 analogs for fluorescent calcium measurements
Authors: Martin VV, Beierlein M, Morgan JL, Rothe A, Gee KR.
Journal: Cell Calcium (2004): 509
Amplitude distribution of calcium sparks in confocal images: theory and studies with an automatic detection method
Authors: Cheng H, Song LS, Shirokova N, Gonzalez A, Lakatta EG, Rios E, Stern MD.
Journal: Biophys J (1999): 606
Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41
Authors: do Ceu Monteiro M, Sansonetty F, Goncalves MJ, O’Connor JE.
Journal: Cytometry (1999): 302
A simple numerical model of calcium spark formation and detection in cardiac myocytes
Authors: Smith GD, Keizer JE, Stern MD, Lederer WJ, Cheng H.
Journal: Biophys J (1998): 15
Monitoring calcium in outer hair cells with confocal microscopy and fluorescence ratios of fluo-3 and fura-red
Authors: Su ZL, Li N, Sun YR, Yang J, Wang IM, Jiang SC.
Journal: Shi Yan Sheng Wu Xue Bao (1998): 323
Loading and localization of Fluo-3 and Fluo-3/AM calcium indicators in sinapis alba root tissue
Authors: Tretyn A, Kado RT, Kendrick RE.
Journal: Folia Histochem Cytobiol (1997): 41
Nucleoplasmic and cytoplasmic differences in the fluorescence properties of the calcium indicator Fluo-3
Authors: Perez-Terzic C, Stehno-Bittel L, Clapham DE.
Journal: Cell Calcium (1997): 275
Detection of a trigger zone of bradykinin-induced fast calcium waves in PC12 neurites
Authors: Reber BF, Schindelholz B.
Journal: Pflugers Arch (1996): 893
Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets
Authors: Greimers R, Trebak M, Moutschen M, Jacobs N, Boniver J.
Journal: Cytometry (1996): 205