nanopartz抗体负荷估计
具有 Nanopartz™ 共价键合聚合物的功能化金纳米颗粒
下表显示了使用 PEG 作为共价聚合物桥的金纳米颗粒上 IgG 的典型负载量。我们的体外聚合物是原来的 2 倍。我们的体内聚合物含量高出约 50%。
球体吸收
部分 # |
直径(纳米) |
峰值SPR(纳米) |
NPS/毫升 |
摩尔浓度 (pM) |
痣 |
IgG抗体/np |
总(和)IgG |
CA11-1.8 |
1.8 |
不适用 |
4.25E+16 |
7.10E+07 |
7.09E-08 |
0.1 |
1,379 |
CA11-2.2 |
2.2 |
不适用 |
2.33E+16 |
3.90E+07 |
3.88E-08 |
0.2 |
1,128 |
CA11-3 |
3 |
不适用 |
9.19E+15 |
1.50E+07 |
1.53E-08 |
0.4 |
827 |
CA11-4 |
4 |
不适用 |
3.88E+15 |
6.50E+06 |
6.46E-09 |
0.6 |
620 |
CA11-5 |
5 |
512 |
1.99E+15 |
3.30E+06 |
3.31E-09 |
1 |
496 |
CA11-10 |
10 |
516 |
2.48E+14 |
4.10E+05 |
4.14E-10 |
4 |
248 |
CA11-15 |
15 |
518 |
7.35E+13 |
1.20E+05 |
1.23E-10 |
9 |
165 |
CA11-20 |
20 |
520 |
3.10E+13 |
5.20E+04 |
5.17E-11 |
16 |
124 |
CA11-25 |
25 |
521 |
1.59E+13 |
2.60E+04 |
2.65E-11 |
25 |
99 |
CA11-30 |
30 |
523 |
9.19E+12 |
1.50E+04 |
1.53E-11 |
36 |
83 |
CA11-35 |
35 |
526 |
5.79E+12 |
9.60E+03 |
9.65E-12 |
49 |
71 |
CA11-40 |
40 |
527 |
3.88E+12 |
6.50E+03 |
6.46E-12 |
64 |
62 |
CA11-45 |
45 |
529 |
2.72E+12 |
4.50E+03 |
4.54E-12 |
81 |
55 |
CA11-50 |
50 |
531 |
1.99E+12 |
3.30E+03 |
3.31E-12 |
100 |
50 |
CA11-55 |
55 |
533 |
1.49E+12 |
2.50E+03 |
2.49E-12 |
121 |
45 |
CA11-60 |
60 |
536 |
1.15E+12 |
1.90E+03 |
1.91E-12 |
144 |
41 |
CA11-65 |
65 |
539 |
9.04E+11 |
1.50E+03 |
1.51E-12 |
169 |
38 |
CA11-70 |
70 |
542 |
7.23E+11 |
1.20E+03 |
1.21E-12 |
196 |
35 |
CA11-75 |
75 |
545 |
5.88E+11 |
9.80E+02 |
9.80E-13 |
225 |
33 |
CA11-80 |
80 |
549 |
4.85E+11 |
8.10E+02 |
8.08E-13 |
256 |
31 |
CA11-85 |
85 |
553 |
4.04E+11 |
6.70E+02 |
6.73E-13 |
289 |
29 |
CA11-90 |
90 |
558 |
3.40E+11 |
5.70E+02 |
5.67E-13 |
324 |
28 |
CA11-95 |
95 |
563 |
2.89E+11 |
4.80E+02 |
4.82E-13 |
361 |
26 |
CA11-100 |
100 |
569 |
2.48E+11 |
4.10E+02 |
4.14E-13 |
400 |
25 |
CA11-150 |
150 |
612 |
7.35E+10 |
1.20E+02 |
1.23E-13 |
900 |
17 号 |
CA11-200 |
200 |
不适用 |
3.10E+10 |
5.20E+01 |
5.17E-14 |
1600 |
12 |
CA11-500 |
500 |
不适用 |
1.99E+09 |
3.30E+00 |
3.31E-15 |
10000 |
5 |
CA11-1000 |
1000 |
不适用 |
2.48E+08 |
4.10E-01 |
4.14E-16 |
40000 |
2 |
CA11-1500 |
1500 |
不适用 |
7.35E+07 |
1.20E-01 |
1.23E-16 |
90000 |
2 |
纳米棒抗体加载
部分 # |
直径(纳米) |
长度(纳米) |
峰值 SPR 波 (nm) |
纵横比 |
从 SPR(澳大利亚)出发 |
IgG抗体/纳米棒 |
总(和)IgG |
CA12-5-808 |
5 |
21 |
808 |
4.2 |
50 |
4 |
251 |
CA12-5-780 |
5 |
19 |
780 |
3.8 |
50 |
4 |
253 |
CA12-5-700 |
5 |
15 |
700 |
3 |
50 |
3 |
260 |
CA12-10-2100 |
10 |
175 |
2100 |
17.5 |
50 |
70 |
118 |
CA12-10-1400 |
10 |
102 |
1400 |
10.2 |
50 |
41 |
120 |
CA12-10-1200 |
10 |
81 |
1200 |
8.1 |
50 |
33 |
121 |
CA12-10-1064 |
10 |
67 |
1064 |
6.7 |
50 |
27 |
122 |
CA12-10-980 |
10 |
59 |
980 |
5.9 |
50 |
23 |
123 |
CA12-10-950 |
10 |
55 |
950 |
5.5 |
50 |
22 |
123 |
CA12-10-900 |
10 |
50 |
900 |
5 |
50 |
20 |
124 |
CA12-10-850 |
10 |
45 |
850 |
4.5 |
50 |
18 |
125 |
CA12-10-808 |
10 |
41 |
808 |
4.1 |
50 |
16 |
126 |
CA12-10-780 |
10 |
38 |
780 |
3.8 |
50 |
15 |
127 |
CA12-10-750 |
10 |
35 |
750 |
3.5 |
50 |
14 |
128 |
CA12-10-700 |
10 |
29 |
700 |
2.9 |
50 |
12 |
130 |
CA12-10-650 |
10 |
24 |
650 |
2.4 |
50 |
10 |
134 |
CA12-10-600 |
10 |
19 |
600 |
1.9 |
50 |
8 |
140 |
CA12-25-1400 |
25 |
245 |
1400 |
9.8 |
50 |
245 |
68 |
CA12-25-1064 |
25 |
137 |
1064 |
5.5 |
50 |
137 |
70 |
CA12-25-980 |
25 |
119 |
980 |
4.8 |
50 |
119 |
71 |
CA12-25-950 |
25 |
102 |
950 |
4.1 |
50 |
102 |
72 |
CA12-25-900 |
25 |
96 |
900 |
3.8 |
50 |
96 |
72 |
CA12-25-850 |
25 |
93 |
850 |
3.7 |
50 |
93 |
73 |
CA12-25-808 |
25 |
90 |
808 |
3.6 |
50 |
90 |
73 |
CA12-25-780 |
25 |
87 |
780 |
3.5 |
50 |
87 |
73 |
CA12-25-750 |
25 |
85 |
750 |
3.4 |
50 |
85 |
73 |
CA12-25-700 |
25 |
75 |
700 |
3 |
50 |
75 |
74 |
CA12-25-650 |
25 |
71 |
650 |
2.8 |
50 |
71 |
75 |
CA12-25-600 |
25 |
57 |
600 |
2.3 |
50 |
57 |
77 |
CA12-25-550 |
25 |
34 |
550 |
1.4 |
50 |
34 |
87 |
CA12-40-1064 |
40 |
208 |
850 |
5.2 |
50 |
333 |
44 |
CA12-40-980 |
40 |
180 |
850 |
4.5 |
50 |
288 |
45 |
CA12-40-850 |
40 |
148 |
850 |
3.7 |
50 |
237 |
45 |
CA12-40-808 |
40 |
134 |
808 |
3.4 |
50 |
214 |
46 |
CA12-40-780 |
40 |
124 |
780 |
3.1 |
50 |
198 |
46 |
CA12-40-750 |
40 |
112 |
750 |
2.8 |
50 |
179 |
47 |
CA12-40-700 |
40 |
92 |
700 |
2.3 |
50 |
147 |
48 |
CA12-40-650 |
40 |
80 |
650 |
2 |
50 |
128 |
50 |
CA12-40-600 |
40 |
68 |
600 |
1.7 |
50 |
109 |
51 |
CA12-40-550 |
40 |
60 |
550 |
1.5 |
50 |
96 |
53 |
CA12-50-1064 |
50 |
245 |
1064 |
4.9 |
50 |
490 |
35 |
CA12-50-808 |
50 |
145 |
808 |
2.9 |
50 |
290 |
37 |
CA12-50-700 |
50 |
110 |
700 |
2.2 |
50 |
220 |
39 |
CA12-50-600 |
50 |
100 |
600 |
2 |
50 |
200 |
40 |
CA12-70-750 |
70 |
120 |
750 |
1.7 |
50 |
336 |
29 |
CA12-70-650 |
70 |
105 |
650 |
1.5 |
50 |
294 |
30 |
CA12-70-550 |
70 |
91 |
550 |
1.3 |
50 |
255 |
32 |