胰凝乳蛋白酶荧光底物MeO-Succ-Arg-Pro-Tyr-AMC-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
胰凝乳蛋白酶荧光底物MeO-Succ-Arg-Pro-Tyr-AMC价格 1386
产品规格

5 mg

产品货号

胰凝乳蛋白酶荧光底物MeO-Succ-Arg-Pro-Tyr-AMC

产品参数
Ex (nm) 341 Em (nm) 441
分子量 819.78 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:胰凝乳蛋白酶荧光底物MeO-Succ-Arg-Pro-Tyr-AMC

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:819.78

溶剂:DMSO

激发波长(nm):351

发射波长(nm):430

 

产品介绍

胰凝乳蛋白酶荧光底物MeO-Succ-Arg-Pro-Tyr-AMC是美国AAT Bioquest生产的荧光底物,MeO-Succ-Arg-Pro-Tyr-AMC是一种类似于胰凝乳蛋白酶的敏感荧光底物。非荧光底物会产生亮蓝色荧光AMC产品,该产品的发射光谱可以通过DAPI滤光片组轻松检测到。它已用于监测角质层糜蛋白酶(SCCE)的蛋白酶活性。金畔生物是AAT Bioquest的中国代理商,为您提供优质的胰凝乳蛋白酶荧光底物MeO-Succ-Arg-Pro-Tyr-AMC。 

点击查看光谱

 

参考文献

A fluorescence lifetime-based assay for protease inhibitor profiling on human kallikrein 7
Authors: Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U.
Journal: J Biomol Screen (2009): 1

A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease
Authors: Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP.
Journal: Anal Biochem (2009): 195

A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates
Authors: Nakata H, Ohtsuki T, Sisido M.
Journal: Anal Biochem (2009): 121

Dual-function probe to detect protease activity for fluorescence measurement and 19F MRI
Authors: Mizukami S, Takikawa R, Sugihara F, Shirakawa M, Kikuchi K.
Journal: Angew Chem Int Ed Engl (2009): 3641

Fluorescence resonance energy transfer-based assay for characterization of hepatitis C virus NS3-4A protease activity in live cells
Authors: Sabariegos R, Picazo F, Domingo B, Franco S, Martinez MA, Llopis J.
Journal: Antimicrob Agents Chemother (2009): 728

Improved detection of ovarian cancer metastases by intraoperative quantitative fluorescence protease imaging in a pre-clinical model
Authors: Sheth RA, Upadhyay R, Stangenberg L, Sheth R, Weissleder R, Mahmood U.
Journal: Gynecol Oncol (2009): 616

Label-free, homogeneous, and fluorescence “turn-on” detection of protease using conjugated polyelectrolytes
Authors: An L, Liu L, Wang S.
Journal: Biomacromolecules (2009): 454

Real-time detection of caspase-3-like protease activation in vivo using fluorescence resonance energy transfer during plant programmed cell death induced by ultraviolet C overexposure
Authors: Zhang L, Xu Q, Xing D, Gao C, Xiong H.
Journal: Plant Physiol (2009): 1773

Real-time monitoring of human enterovirus (HEV)-infected cells and anti-HEV 3C protease potency by fluorescence resonance energy transfer
Authors: Tsai MT, Cheng YH, Liu YN, Liao NC, Lu WW, Kung SH.
Journal: Antimicrob Agents Chemother (2009): 748

[Prokaryotic expression and Identification of dual-fluorescence fusion proteins of small ubiquitin-like modifier and sentrin-specific protease]
Authors: Wang X, Guo J, Wang J, Li S, Sun L, Lu J.
Journal: Sheng Wu Gong Cheng Xue Bao (2009): 701

蛋白酶荧光底物Benzoyl-Arg-AMC-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
蛋白酶荧光底物Benzoyl-Arg-AMC价格 1386
产品规格

5 mg

产品货号

蛋白酶荧光底物Benzoyl-Arg-AMC

产品参数
Ex (nm) 341 Em (nm) 441
分子量 549.50 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:蛋白酶荧光底物Benzoyl-Arg-AMC

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:549.50

外观:橙色粉末

溶剂:DMSO

激发波长(nm):351

发射波长(nm):430

 

产品介绍

蛋白酶荧光底物Benzoyl-Arg-AMC是美国AAT Bioquest生产的荧光底物,该苯甲酰-Arg-AMC是一种荧光氨基肽酶和内肽酶底物,用于检测人α-凝血酶的酶活性。 非荧光底物会产生亮蓝色荧光AMC产品,该产品的发射光谱可以通过DAPI滤光片组轻松检测到。金畔生物是AAT Bioquest的中国代理商,为您提供优质的蛋白酶荧光底物Benzoyl-Arg-AMC。 

点击查看光谱

 

参考文献

A fluorescence lifetime-based assay for protease inhibitor profiling on human kallikrein 7
Authors: Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U.
Journal: J Biomol Screen (2009): 1

A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease
Authors: Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP.
Journal: Anal Biochem (2009): 195

A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates
Authors: Nakata H, Ohtsuki T, Sisido M.
Journal: Anal Biochem (2009): 121

Dual-function probe to detect protease activity for fluorescence measurement and 19F MRI
Authors: Mizukami S, Takikawa R, Sugihara F, Shirakawa M, Kikuchi K.
Journal: Angew Chem Int Ed Engl (2009): 3641

Fluorescence resonance energy transfer-based assay for characterization of hepatitis C virus NS3-4A protease activity in live cells
Authors: Sabariegos R, Picazo F, Domingo B, Franco S, Martinez MA, Llopis J.
Journal: Antimicrob Agents Chemother (2009): 728

Improved detection of ovarian cancer metastases by intraoperative quantitative fluorescence protease imaging in a pre-clinical model
Authors: Sheth RA, Upadhyay R, Stangenberg L, Sheth R, Weissleder R, Mahmood U.
Journal: Gynecol Oncol (2009): 616

Label-free, homogeneous, and fluorescence “turn-on” detection of protease using conjugated polyelectrolytes
Authors: An L, Liu L, Wang S.
Journal: Biomacromolecules (2009): 454

Real-time detection of caspase-3-like protease activation in vivo using fluorescence resonance energy transfer during plant programmed cell death induced by ultraviolet C overexposure
Authors: Zhang L, Xu Q, Xing D, Gao C, Xiong H.
Journal: Plant Physiol (2009): 1773

Real-time monitoring of human enterovirus (HEV)-infected cells and anti-HEV 3C protease potency by fluorescence resonance energy transfer
Authors: Tsai MT, Cheng YH, Liu YN, Liao NC, Lu WW, Kung SH.
Journal: Antimicrob Agents Chemother (2009): 748

[Prokaryotic expression and Identification of dual-fluorescence fusion proteins of small ubiquitin-like modifier and sentrin-specific protease]
Authors: Wang X, Guo J, Wang J, Li S, Sun L, Lu J.
Journal: Sheng Wu Gong Cheng Xue Bao (2009): 701

蛋白酶荧光底物Bis-Arg-rhodamine 110-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
蛋白酶荧光底物Bis-Arg-rhodamine 110价格 4245
产品规格

1 mg

产品货号

蛋白酶荧光底物Bis-Arg-rhodamine 110

产品参数
Ex (nm) 500 Em (nm) 522
分子量 1098.81 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:Bis-Arg-罗丹明110

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:1098.81

外观:固体

溶剂:DMSO

激发波长(nm):498

发射波长(nm):520

 

产品介绍

Bis-Arg-罗丹明110是一种氨基肽酶和内肽酶荧光底物,是美国AAT Bioquest研发生产的罗丹明类染料,用于检测人α-凝血酶的酶活性。非荧光底物会产生亮绿色的荧光罗丹明产物,并且可以被FITC滤波片组轻松检测到。金畔生物是AAT Bioquest的中国代理商,为您提供优质的罗丹明染料。 

点击查看光谱

 

试剂应用文献

NMR reveals a dynamic allosteric pathway in thrombin
Authors: H, undefined and ley, L. D., Fuglestad, B., Stearns, K., Tonelli, M., Fenwick, R. B., Markwick, P. R., Komives, E. A.
Journal: Sci Rep (2017): 39575
 
Continuous-flow injection microfluidic thrombin assays: The effect of binding kinetics on observed enzyme inhibition
Authors: Ramjee, M. K., Patel, S.
Journal: Anal Biochem (2017): 38-46
 
Inherited Anti-Thrombin Deficiency in A Malay-Malaysian Family: A Missense Mutation at Nucleotide g.13267C>A aka anti-thrombin Budapest 5 (p.Pro439Thr) of the SERPINC 1 gene
Authors: Norlelawati, A. T., Rusmawati, I., Naznin, M., Nur Nadia, O., Rizqan Aizzani, R., Noraziana, A. W.
Journal: Med J Malaysia (2014): 27-30
 
The role of protein S in the activation of thrombin activatable fibrinolysis inhibitor (TAFI) and regulation of fibrinolysis
Authors: Mosnier, L. O., Meijers, J. C., Bouma, B. N.
Journal: Thromb Haemost (2001): 1040-6
 
Protein C inhibitor regulates the thrombin-thrombomodulin complex in the up- and down regulation of TAFI activation
Authors: Mosnier, L. O., Elisen, M. G., Bouma, B. N., Meijers, J. C.
Journal: Thromb Haemost (2001): 1057-64
 
Thrombin activatable fibrinolysis inhibitor (TAFI) does not inhibit in vitro thrombolysis by pharmacological concentrations of t-PA
Authors: Colucci, M., D’Aprile, A. M., Italia, A., Gresele, P., Morser, J., Semeraro, N.
Journal: Thromb Haemost (2001): 661-6
 
Synergistic actions of a thrombin-derived synthetic peptide and a thrombin receptor-activating peptide in stimulating fibroblast mitogenesis
Authors: Hollenberg, M. D., Mokashi, S., Leblond, L., DiMaio, J.
Journal: J Cell Physiol (1996): 491-6
 
Efficient production of the C-terminal domain of secretory leukoprotease inhibitor as a thrombin-cleavable fusion protein in Escherichia coli
Authors: Masuda, K., Kamimura, T., Kanesaki, M., Ishii, K., Imaizumi, A., Sugiyama, T., Suzuki, Y., Ohtsuka, E.
Journal: Protein Eng (1996): 101-6
 
Activation of thrombin-inactivated single-chain urokinase-type plasminogen activator by dipeptidyl peptidase I (cathepsin C)
Authors: Naul, undefined and , U., Rijken, D. C.
Journal: Eur J Biochem (1994): 497-501
 
Amidinated thrombin: preparation and peptidase activity
Authors: Kang, E. P.
Journal: Thromb Res (1978): 177-80

发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素价格 7092
产品规格

1 mg

产品货号

发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素

产品参数
Ex (nm) 362 Em (nm) 499
分子量 823.86 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:823.86

溶剂:DMSO

激发波长(nm):N/A

发射波长(nm):560

 

产品介绍

发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素是美国AAT Bioquest生产的荧光染料,这种基于氨基荧光素的探针是补体成分C3 / C5转化酶,凝血因子Xa和大豆胰蛋白酶样酶的发光底物。 它还被来自人红细胞的高分子量硫醇蛋白酶macropain水解。 可以使用我们的荧光素酶测定试剂盒检测蛋白酶释放的D-氨基荧光素。 苯甲酰基-Leu-Gly-Arg-Aminoluciferin比广泛使用的荧光AMC底物Boc-Leu-Gly-Arg-AMC灵敏得多。金畔生物是AAT Bioquest的中国代理商,为您提供优质的发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素。 

 

试剂应用文献

Improved bioluminescence-based endotoxin measurement method using a salt-resistant luciferase mutant
Authors: Yawata, Satoshi and Noda, Kenichi and Shimomura, Ai and Kuroda, Akio
Journal: Analytical Biochemistry (2021): 114408
 
Endotoxin assay by bioluminescence using mutant firefly luciferase
Authors: Noda, Kenichi and Goto, Hitoshi and Murakami, Yuji and Ahmed, Abo Bakr F and Kuroda, Akio
Journal: Analytical biochemistry (2010): 152–155

 

参考文献

Identification of mutant firefly luciferases that efficiently utilize aminoluciferins
Authors: Harwood KR, Mofford DM, Reddy GR, Miller SC.
Journal: Chem Biol (2011): 1649
 
In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation
Authors: Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.
Journal: Apoptosis (2011): 198
 
Aminoluciferins as functional bioluminogenic substrates of firefly luciferase
Authors: Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.
Journal: Chem Asian J (2011): 1800
 
Noninvasive molecular imaging of apoptosis in vivo using a modified firefly luciferase substrate, Z-DEVD-aminoluciferin
Authors: Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.
Journal: Cell Death Differ (2010): 1003
 
Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase
Authors: Reddy, G. R.; Thompson, W. C.; Miller, S. C.
Journal: J Am Chem Soc (2010): 13586-7
 
Cell-based bioluminescent assays for all three proteasome activities in a homogeneous format
Authors: Moravec RA, O’Brien MA, Daily WJ, Scurria MA, Bernad L, Riss TL.
Journal: Anal Biochem (2009): 294
 
In vivo bioluminescence imaging of furin activity in breast cancer cells using bioluminogenic substrates
Authors: Dragulescu-Andrasi A, Liang G, Rao J.
Journal: Bioconjug Chem (2009): 1660
 
Homogeneous, bioluminescent proteasome assays
Authors: O’Brien MA, Moravec RA, Riss TL, Bulleit RF.
Journal: Methods Mol Biol (2008): 163
 
N-Alkylated 6′-aminoluciferins are bioluminescent substrates for Ultra-Glo and QuantiLum luciferase: new potential scaffolds for bioluminescent assays
Authors: Woodroofe CC, Shultz JW, Wood MG, Osterman J, Cali JJ, Daily WJ, Meisenheimer PL, Klaubert DH.
Journal: Biochemistry (2008): 10383
 
A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers
Authors: Niles AL, Moravec RA, Eric Hesselberth P, Scurria MA, Daily WJ, Riss TL.
Journal: Anal Biochem (2007): 197

 

相关产品

产品名称 货号
荧光底物BOC-Leu-Gly-Arg-氨基荧光素 Cat#13001

发光底物BOC-Leu-Gly-Arg-氨基荧光素-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
发光底物BOC-Leu-Gly-Arg-氨基荧光素价格 7092
产品规格

1 mg

产品货号

发光底物BOC-Leu-Gly-Arg-氨基荧光素

产品参数
Ex (nm) 362 Em (nm) 499
分子量 819.87 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:发光底物BOC-Leu-Gly-Arg-氨基荧光素

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:819.87

溶剂:DMSO

激发波长(nm):N/A

发射波长(nm):560

 

产品介绍

发光底物BOC-Leu-Gly-Arg-氨基荧光素是美国AAT Bioquest生产的荧光染料,这种基于氨基荧光素的探针是补体成分C3 / C5转化酶,凝血因子Xa和大豆胰蛋白酶样酶的发光底物。 它也被来自人类红细胞的高分子量巯基蛋白酶macropain水解。 可以使用我们的萤光素酶测定试剂盒检测蛋白酶释放的D-氨基荧光素。BOC-Leu-Gly-Arg-Aminoluciferin比广泛使用的荧光AMC底物Boc-Leu-Gly-Arg-AMC敏感得多。金畔生物是AAT Bioquest的中国代理商,为您提供优质的发光底物BOC-Leu-Gly-Arg-氨基荧光素。 

 

试剂应用文献

Endotoxin assay by bioluminescence using mutant firefly luciferase
Authors: Kenichi Noda, Hitoshi Goto, Yuji Murakami, Abo Bakr F Ahmed, Akio Kuroda
Journal: Analytical biochemistry (2010): 152–155

 

参考文献

Identification of mutant firefly luciferases that efficiently utilize aminoluciferins
Authors: Harwood KR, Mofford DM, Reddy GR, Miller SC.
Journal: Chem Biol (2011): 1649
 
In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation
Authors: Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.
Journal: Apoptosis (2011): 198
 
Aminoluciferins as functional bioluminogenic substrates of firefly luciferase
Authors: Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.
Journal: Chem Asian J (2011): 1800
 
Noninvasive molecular imaging of apoptosis in vivo using a modified firefly luciferase substrate, Z-DEVD-aminoluciferin
Authors: Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.
Journal: Cell Death Differ (2010): 1003
 
Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase
Authors: Reddy, G. R.; Thompson, W. C.; Miller, S. C.
Journal: J Am Chem Soc (2010): 13586-7
 
Cell-based bioluminescent assays for all three proteasome activities in a homogeneous format
Authors: Moravec RA, O’Brien MA, Daily WJ, Scurria MA, Bernad L, Riss TL.
Journal: Anal Biochem (2009): 294
 
In vivo bioluminescence imaging of furin activity in breast cancer cells using bioluminogenic substrates
Authors: Dragulescu-Andrasi A, Liang G, Rao J.
Journal: Bioconjug Chem (2009): 1660
 
Homogeneous, bioluminescent proteasome assays
Authors: O’Brien MA, Moravec RA, Riss TL, Bulleit RF.
Journal: Methods Mol Biol (2008): 163
 
N-Alkylated 6′-aminoluciferins are bioluminescent substrates for Ultra-Glo and QuantiLum luciferase: new potential scaffolds for bioluminescent assays
Authors: Woodroofe CC, Shultz JW, Wood MG, Osterman J, Cali JJ, Daily WJ, Meisenheimer PL, Klaubert DH.
Journal: Biochemistry (2008): 10383
 
A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers
Authors: Niles AL, Moravec RA, Eric Hesselberth P, Scurria MA, Daily WJ, Riss TL.
Journal: Anal Biochem (2007): 197

 

相关产品

产品名称 货号
荧光底物Benzoyl-Leu-Gly-Arg-氨基荧光素 Cat#13000

发光底物BOC-Val-Pro-Arg-氨基荧光素-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
发光底物BOC-Val-Pro-Arg-氨基荧光素价格 7092
产品规格

1 mg

产品货号

发光底物BOC-Val-Pro-Arg-氨基荧光素

产品参数
Ex (nm) 362 Em (nm) 499
分子量 845.91 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:BOC-Val-Pro-Arg-氨基荧光素

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:845.91

溶剂:DMSO

 

产品介绍

        BOC-Val-Pro-Arg-氨基荧光素是美国AAT Bioquest生产的荧光底物,BOC-Val-Pro-Arg-氨基荧光素是α-凝血酶的敏感发光底物。不发光底物产生明亮的发光产物氨基荧光素,其可以用荧光素酶容易地检测。该底物也已用于MRSA(耐甲氧西林金黄色葡萄球菌)的快速检测。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的BOC-Val-Pro-Arg-氨基荧光素。 

 

试剂应用文献

Endotoxin assay by bioluminescence using mutant firefly luciferase
Authors: Kenichi Noda, Hitoshi Goto, Yuji Murakami, Abo Bakr F Ahmed, Akio Kuroda
Journal: Analytical biochemistry (2010): 152–155

 

参考文献

Identification of mutant firefly luciferases that efficiently utilize aminoluciferins
Authors: Harwood KR, Mofford DM, Reddy GR, Miller SC.
Journal: Chem Biol (2011): 1649
 
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胰凝乳蛋白酶荧光底物BOC-Val-Pro-Arg-AMC Cat#13461

Z-Gly-Arg-thiobenzyl ester03SB007-CF


Z-Gly-Arg-thiobenzyl ester

简要描述:Z-Gly-Arg-thiobenzyl ester,产品型号:03SB007-CF
肽硫代苄酯是丝氨酸和半胱氨酸蛋白酶非常敏感的底物。Plasmogen 激活剂丝氨酸蛋白酶的显色底物

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品牌 其他品牌 供货周期 一个月
应用领域 医疗卫生,环保,化工,生物产业,综合

Z-Gly-Arg-thiobenzyl ester,产品型号:03SB007-CF。

Z-Gly-Arg-thiobenzyl ester,描述:

肽硫代苄酯是丝氨酸和半胱氨酸蛋白酶非常敏感的底物。硫醇离去基团通过与硫醇试剂如 5,5'-二硫代双(2-硝基苯甲酸)(DTNB 或 Leman 试剂)或 4,4'-二硫代二吡啶反应来检测。然后通过监测 4-硫代吡啶酮的形成 (E324nm = 19,800 M -1 cm -1 ) 在 324 nm 或使用 DTNB 作为硫醇试剂在 405 nm (E405nm = 13,260 M -1 cm – 1 ) 对于 2-nitro-5-thiobenzoate 阴离子 TNBS。Plasmogen 激活剂丝氨酸蛋白酶的显色底物


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