蛋白酶荧光底物Benzoyl-Arg-AMC-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
蛋白酶荧光底物Benzoyl-Arg-AMC价格 1386
产品规格

5 mg

产品货号

蛋白酶荧光底物Benzoyl-Arg-AMC

产品参数
Ex (nm) 341 Em (nm) 441
分子量 549.50 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:蛋白酶荧光底物Benzoyl-Arg-AMC

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:549.50

外观:橙色粉末

溶剂:DMSO

激发波长(nm):351

发射波长(nm):430

 

产品介绍

蛋白酶荧光底物Benzoyl-Arg-AMC是美国AAT Bioquest生产的荧光底物,该苯甲酰-Arg-AMC是一种荧光氨基肽酶和内肽酶底物,用于检测人α-凝血酶的酶活性。 非荧光底物会产生亮蓝色荧光AMC产品,该产品的发射光谱可以通过DAPI滤光片组轻松检测到。金畔生物是AAT Bioquest的中国代理商,为您提供优质的蛋白酶荧光底物Benzoyl-Arg-AMC。 

点击查看光谱

 

参考文献

A fluorescence lifetime-based assay for protease inhibitor profiling on human kallikrein 7
Authors: Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U.
Journal: J Biomol Screen (2009): 1

A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease
Authors: Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP.
Journal: Anal Biochem (2009): 195

A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates
Authors: Nakata H, Ohtsuki T, Sisido M.
Journal: Anal Biochem (2009): 121

Dual-function probe to detect protease activity for fluorescence measurement and 19F MRI
Authors: Mizukami S, Takikawa R, Sugihara F, Shirakawa M, Kikuchi K.
Journal: Angew Chem Int Ed Engl (2009): 3641

Fluorescence resonance energy transfer-based assay for characterization of hepatitis C virus NS3-4A protease activity in live cells
Authors: Sabariegos R, Picazo F, Domingo B, Franco S, Martinez MA, Llopis J.
Journal: Antimicrob Agents Chemother (2009): 728

Improved detection of ovarian cancer metastases by intraoperative quantitative fluorescence protease imaging in a pre-clinical model
Authors: Sheth RA, Upadhyay R, Stangenberg L, Sheth R, Weissleder R, Mahmood U.
Journal: Gynecol Oncol (2009): 616

Label-free, homogeneous, and fluorescence “turn-on” detection of protease using conjugated polyelectrolytes
Authors: An L, Liu L, Wang S.
Journal: Biomacromolecules (2009): 454

Real-time detection of caspase-3-like protease activation in vivo using fluorescence resonance energy transfer during plant programmed cell death induced by ultraviolet C overexposure
Authors: Zhang L, Xu Q, Xing D, Gao C, Xiong H.
Journal: Plant Physiol (2009): 1773

Real-time monitoring of human enterovirus (HEV)-infected cells and anti-HEV 3C protease potency by fluorescence resonance energy transfer
Authors: Tsai MT, Cheng YH, Liu YN, Liao NC, Lu WW, Kung SH.
Journal: Antimicrob Agents Chemother (2009): 748

[Prokaryotic expression and Identification of dual-fluorescence fusion proteins of small ubiquitin-like modifier and sentrin-specific protease]
Authors: Wang X, Guo J, Wang J, Li S, Sun L, Lu J.
Journal: Sheng Wu Gong Cheng Xue Bao (2009): 701

发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素价格 7092
产品规格

1 mg

产品货号

发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素

产品参数
Ex (nm) 362 Em (nm) 499
分子量 823.86 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:823.86

溶剂:DMSO

激发波长(nm):N/A

发射波长(nm):560

 

产品介绍

发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素是美国AAT Bioquest生产的荧光染料,这种基于氨基荧光素的探针是补体成分C3 / C5转化酶,凝血因子Xa和大豆胰蛋白酶样酶的发光底物。 它还被来自人红细胞的高分子量硫醇蛋白酶macropain水解。 可以使用我们的荧光素酶测定试剂盒检测蛋白酶释放的D-氨基荧光素。 苯甲酰基-Leu-Gly-Arg-Aminoluciferin比广泛使用的荧光AMC底物Boc-Leu-Gly-Arg-AMC灵敏得多。金畔生物是AAT Bioquest的中国代理商,为您提供优质的发光底物Benzoyl-Leu-Gly-Arg-氨基荧光素。 

 

试剂应用文献

Improved bioluminescence-based endotoxin measurement method using a salt-resistant luciferase mutant
Authors: Yawata, Satoshi and Noda, Kenichi and Shimomura, Ai and Kuroda, Akio
Journal: Analytical Biochemistry (2021): 114408
 
Endotoxin assay by bioluminescence using mutant firefly luciferase
Authors: Noda, Kenichi and Goto, Hitoshi and Murakami, Yuji and Ahmed, Abo Bakr F and Kuroda, Akio
Journal: Analytical biochemistry (2010): 152–155

 

参考文献

Identification of mutant firefly luciferases that efficiently utilize aminoluciferins
Authors: Harwood KR, Mofford DM, Reddy GR, Miller SC.
Journal: Chem Biol (2011): 1649
 
In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation
Authors: Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.
Journal: Apoptosis (2011): 198
 
Aminoluciferins as functional bioluminogenic substrates of firefly luciferase
Authors: Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.
Journal: Chem Asian J (2011): 1800
 
Noninvasive molecular imaging of apoptosis in vivo using a modified firefly luciferase substrate, Z-DEVD-aminoluciferin
Authors: Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.
Journal: Cell Death Differ (2010): 1003
 
Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase
Authors: Reddy, G. R.; Thompson, W. C.; Miller, S. C.
Journal: J Am Chem Soc (2010): 13586-7
 
Cell-based bioluminescent assays for all three proteasome activities in a homogeneous format
Authors: Moravec RA, O’Brien MA, Daily WJ, Scurria MA, Bernad L, Riss TL.
Journal: Anal Biochem (2009): 294
 
In vivo bioluminescence imaging of furin activity in breast cancer cells using bioluminogenic substrates
Authors: Dragulescu-Andrasi A, Liang G, Rao J.
Journal: Bioconjug Chem (2009): 1660
 
Homogeneous, bioluminescent proteasome assays
Authors: O’Brien MA, Moravec RA, Riss TL, Bulleit RF.
Journal: Methods Mol Biol (2008): 163
 
N-Alkylated 6′-aminoluciferins are bioluminescent substrates for Ultra-Glo and QuantiLum luciferase: new potential scaffolds for bioluminescent assays
Authors: Woodroofe CC, Shultz JW, Wood MG, Osterman J, Cali JJ, Daily WJ, Meisenheimer PL, Klaubert DH.
Journal: Biochemistry (2008): 10383
 
A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers
Authors: Niles AL, Moravec RA, Eric Hesselberth P, Scurria MA, Daily WJ, Riss TL.
Journal: Anal Biochem (2007): 197

 

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荧光底物BOC-Leu-Gly-Arg-氨基荧光素 Cat#13001