样品实验方案

操作步骤

以下协议适用于大多数细胞类型。这些条件需要调整每种细胞类型和实验系统。生长培养基，细胞密度，其他细胞类型和因子的存在可能影响染色。玻璃器皿上的残留洗涤剂也可能影响许多生物的染色，并导致在有或没有细胞存在的溶液中出现明亮染色的物质。

稀释MycoLight Green JJ98和JJ99时使用塑料管，因为稀释的污渍粘附在玻璃上。通常，在不含磷酸盐的缓冲液中获得佳结果。

1.培养物中的贴壁细胞可以在盖玻片上原位染色。通过离心和悬浮液沉淀细胞重悬于缓冲盐溶液或水中。

2.用非磷酸盐缓冲液（如Hepes缓冲液或您选择的缓冲液）稀释MycoLight Green JJ98或JJ99。添加MycoLight Green JJ98。使用下表1中列出的浓度作为指导。在的实验中，在整个建议范围内尝试几种染料浓度，以确定产生染色的浓度。

表格1 用MycoLight Green JJ98染色细胞的建议条件

3.染色的真核细胞通常显示弥漫性细胞质染色以及核染色。 特别是经常观察到MycoLight Green JJ98和JJ99对核内体的强烈荧光染色。

参考文献

A loop-mediated isothermal amplification (LAMP) assay for Strongyloides stercoralis in stool that uses a visual detection method with SYTO-82 fluorescent dye
Authors: Watts MR, James G, Sultana Y, Ginn AN, Outhred AC, Kong F, Verweij JJ, Iredell JR, Chen SC, Lee R.
Journal: Am J Trop Med Hyg (2014): 306

A new triplex real time PCR which distinguishes between MRSA, MSSA, and mecA coagulase negative strains by means of melting point analysis using SYTO 9
Authors: Weidner J, Cassens U, Gohde W, Wullenweber J, Greve B.
Journal: Clin Lab (2013): 795

Compatibility of SYTO 13 and Hoechst 33342 for longitudinal imaging of neuron viability and cell death
Authors: Hubbard KS, Gut IM, Scheeler SM, Lyman ME, McNutt PM.
Journal: BMC Res Notes (2012): 437

Evaluation of Pseudomonas aeruginosa (PAO1) adhesion to human alveolar epithelial cells A549 using SYTO 9 dye
Authors: Larrosa M, Truchado P, Espin JC, Tomas-Barberan FA, Allende A, Garcia-Conesa MT.
Journal: Mol Cell Probes (2012): 121

Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes
Authors: Wlodkowic D, Skommer J, Darzynkiewicz Z.
Journal: Methods Mol Biol (2011): 81

SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR
Authors: Eischeid AC.
Journal: BMC Res Notes (2011): 263

Detection of methicillin-resistant Staphylococcus aureus using double duplex real-time PCR and dye Syto 9
Authors: Seputiene V, Vilkoicaite A, Armalyte J, Pavilonis A, Suziedeliene E.
Journal: Folia Microbiol (Praha) (2010): 502

Use of SYTO 13, a fluorescent dye binding nucleic acids, for the detection of microparticles in in vitro systems
Authors: Ullal AJ, Pisetsky DS, Reich CF, 3rd.
Journal: Cytometry A (2010): 294

Assessment of acridine orange and SYTO 16 for in vivo imaging of the peritoneal tissues in mice
Authors: Udovich JA, Besselsen DG, Gmitro AF.
Journal: J Microsc (2009): 124

Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometry
Authors: Wlodkowic D, Skommer J, Faley S, Darzynkiewicz Z, Cooper JM.
Journal: Exp Cell Res (2009): 1706

MycoLight Green JJ99核酸染料是美国AAT Bioquest生产的核酸染料，MycoLight 绿色JJ99染色是一种绿色荧光核和染色体复染剂，对原核细胞和真核细胞膜均具有渗透性。MycoLight Green JJ98染料对DNA具有高亲和力，并且在与接近488 nm氩激光线的激发大值和~500 nm处的荧光发射大值结合时显示出增强的荧光。MycoLight Green JJ99染色剂特别适用于细菌检测的核复染剂，因为它可以染色活的和死的革兰氏阳性菌和革兰氏阴性菌。它是SYTO®9（SYTO®是Invitrogen的商标）的替代品。金畔生物是AAT Bioquest的中国代理商，为您提供优质的MycoLight Green JJ99核酸染料。 

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样品实验方案

操作步骤

以下协议适用于大多数细胞类型。这些条件需要调整每种细胞类型和实验系统。生长培养基，细胞密度，其他细胞类型和因子的存在可能影响染色。玻璃器皿上的残留洗涤剂也可能影响许多生物的染色，并导致在有或没有细胞存在的溶液中出现明亮染色的物质。

稀释MycoLight Green JJ98和JJ99时使用塑料管，因为稀释的污渍粘附在玻璃上。通常，在不含磷酸盐的缓冲液中获得佳结果。

1.培养物中的贴壁细胞可以在盖玻片上原位染色。通过离心和悬浮液沉淀细胞重悬于缓冲盐溶液或水中。

2.用非磷酸盐缓冲液（如Hepes缓冲液或您选择的缓冲液）稀释MycoLight Green JJ98或JJ99。添加MycoLight Green JJ98。使用下表1中列出的浓度作为指导。在的实验中，在整个建议范围内尝试几种染料浓度，以确定产生染色的浓度。

表格1 用MycoLight Green JJ98染色细胞的建议条件

3.染色的真核细胞通常显示弥漫性细胞质染色以及核染色。 特别是经常观察到MycoLight Green JJ98和JJ99对核内体的强烈荧光染色。

参考文献

A loop-mediated isothermal amplification (LAMP) assay for Strongyloides stercoralis in stool that uses a visual detection method with SYTO-82 fluorescent dye
Authors: Watts MR, James G, Sultana Y, Ginn AN, Outhred AC, Kong F, Verweij JJ, Iredell JR, Chen SC, Lee R.
Journal: Am J Trop Med Hyg (2014): 306

A new triplex real time PCR which distinguishes between MRSA, MSSA, and mecA coagulase negative strains by means of melting point analysis using SYTO 9
Authors: Weidner J, Cassens U, Gohde W, Wullenweber J, Greve B.
Journal: Clin Lab (2013): 795

Compatibility of SYTO 13 and Hoechst 33342 for longitudinal imaging of neuron viability and cell death
Authors: Hubbard KS, Gut IM, Scheeler SM, Lyman ME, McNutt PM.
Journal: BMC Res Notes (2012): 437

Evaluation of Pseudomonas aeruginosa (PAO1) adhesion to human alveolar epithelial cells A549 using SYTO 9 dye
Authors: Larrosa M, Truchado P, Espin JC, Tomas-Barberan FA, Allende A, Garcia-Conesa MT.
Journal: Mol Cell Probes (2012): 121

Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes
Authors: Wlodkowic D, Skommer J, Darzynkiewicz Z.
Journal: Methods Mol Biol (2011): 81

SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR
Authors: Eischeid AC.
Journal: BMC Res Notes (2011): 263

Detection of methicillin-resistant Staphylococcus aureus using double duplex real-time PCR and dye Syto 9
Authors: Seputiene V, Vilkoicaite A, Armalyte J, Pavilonis A, Suziedeliene E.
Journal: Folia Microbiol (Praha) (2010): 502

Use of SYTO 13, a fluorescent dye binding nucleic acids, for the detection of microparticles in in vitro systems
Authors: Ullal AJ, Pisetsky DS, Reich CF, 3rd.
Journal: Cytometry A (2010): 294

Assessment of acridine orange and SYTO 16 for in vivo imaging of the peritoneal tissues in mice
Authors: Udovich JA, Besselsen DG, Gmitro AF.
Journal: J Microsc (2009): 124

Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometry
Authors: Wlodkowic D, Skommer J, Faley S, Darzynkiewicz Z, Cooper JM.
Journal: Exp Cell Res (2009): 1706