产品基本信息

产品名称：核酸染料LDS 751

CAS：181885-68-7

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：471.98

外观：深绿色粉末

溶剂：DMSO

激发波长(nm)：561

发射波长(nm)：712

产品介绍

LDS 751是一种渗透性细胞核酸染料，它在dsDNA上在〜543 nm处具有峰值激发，但可以用488 nm激光很好地激发。它是DRAQ 5 的替代产品，由于其大的大波长发射波长（〜712 nm），可用于多色分析。与LDS 751的dsDNA结合后，荧光增强程度约为20倍。LDS-751不进入细胞核，并与线粒体膜结合。因此，当使用LDS 751分析活细胞和使用LDS-751探针检测细胞时需要清楚，它不可用于细胞核检测。噻唑橙（RNA染料）和LDS-751（DNA染料）组合物已被用于将网织红细胞与其他细胞群分离。 LDS 751也已用于从有核细胞中分离红细胞。

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参考文献

Exciton-controlled fluorescence: application to hybridization-sensitive fluorescent DNA probe
Authors: Okamoto A, Ikeda S, Kubota T, Yuki M, Yanagisawa H.
Journal: Nucleic Acids Symp Ser (Oxf) (2009): 49

Hybridization-sensitive fluorescent probe for long-term monitoring of intracellular RNA
Authors: Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A.
Journal: Bioconjug Chem (2009): 1256

Interaction of LDS-751 with the drug-binding site of P-glycoprotein: a Trp fluorescence steady-state and lifetime study
Authors: Lugo MR, Sharom FJ.
Journal: Arch Biochem Biophys (2009): 17

Flow cytometric assessment of homeostatic aging of reticulocytes in rats
Authors: Wiczling P, Krzyzanski W.
Journal: Exp Hematol (2008): 119

Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry
Authors: Maes ML, Davidson LB, McDonagh PF, Ritter LS.
Journal: J Immunol Methods (2007): 79

Determination of the formation of dark state via depleted spontaneous emission in a complex solvated molecule
Authors: Guo X, Wang S, Xia A, Su H.
Journal: J Phys Chem A (2007): 5800

Cell cycle synchronization of Cupriavidus necator by continuous phasing measured via flow cytometry
Authors: Fritsch M, Starruss J, Loesche A, Mueller S, Bley T.
Journal: Biotechnol Bioeng (2005): 635

Exciton mobility and trapping in a MALDI matrix
Authors: Setz PD, Knochenmuss R.
Journal: J Phys Chem A (2005): 4030

Interaction of LDS-751 and rhodamine 123 with P-glycoprotein: evidence for simultaneous binding of both drugs
Authors: Lugo MR, Sharom FJ.
Journal: Biochemistry (2005): 14020

Quantitative evaluation of isothiocyanates as substrates and inhibitors of P-glycoprotein
Authors: Barecki-Roach M, Wang EJ, Johnson WW.
Journal: J Pharm Pharmacol (2003): 1251

染色细胞分析方案

注意：以下协议适用于大多数细胞类型。 生长培养基，细胞密度，其他细胞类型和因子的存在可能影响染色。 玻璃器皿上的残留洗涤剂也可能影响许多生物的染色，并导致在有或没有细胞存在的溶液中出现明亮染色的物质。

1.制备5-10 mM DMSO储备溶液。

2.向细胞（悬浮细胞或贴壁细胞）中加入1至10μM，并将细胞染色15至60分钟。 在初的实验中，好尝试几种染料浓度来确定产生所需结果的佳浓度。 高染料浓度倾向于引起其他细胞结构的非特异性染色。

3.用荧光显微镜，荧光酶标仪或流式细胞仪直接分析细胞染色。

参考文献

Exciton-controlled fluorescence: application to hybridization-sensitive fluorescent DNA probe
Authors: Okamoto A, Ikeda S, Kubota T, Yuki M, Yanagisawa H.
Journal: Nucleic Acids Symp Ser (Oxf) (2009): 49

Hybridization-sensitive fluorescent probe for long-term monitoring of intracellular RNA
Authors: Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A.
Journal: Bioconjug Chem (2009): 1256

Interaction of LDS-751 with the drug-binding site of P-glycoprotein: a Trp fluorescence steady-state and lifetime study
Authors: Lugo MR, Sharom FJ.
Journal: Arch Biochem Biophys (2009): 17

Flow cytometric assessment of homeostatic aging of reticulocytes in rats
Authors: Wiczling P, Krzyzanski W.
Journal: Exp Hematol (2008): 119

Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry
Authors: Maes ML, Davidson LB, McDonagh PF, Ritter LS.
Journal: J Immunol Methods (2007): 79

Determination of the formation of dark state via depleted spontaneous emission in a complex solvated molecule
Authors: Guo X, Wang S, Xia A, Su H.
Journal: J Phys Chem A (2007): 5800

Cell cycle synchronization of Cupriavidus necator by continuous phasing measured via flow cytometry
Authors: Fritsch M, Starruss J, Loesche A, Mueller S, Bley T.
Journal: Biotechnol Bioeng (2005): 635

Exciton mobility and trapping in a MALDI matrix
Authors: Setz PD, Knochenmuss R.
Journal: J Phys Chem A (2005): 4030

Interaction of LDS-751 and rhodamine 123 with P-glycoprotein: evidence for simultaneous binding of both drugs
Authors: Lugo MR, Sharom FJ.
Journal: Biochemistry (2005): 14020

Quantitative evaluation of isothiocyanates as substrates and inhibitors of P-glycoprotein
Authors: Barecki-Roach M, Wang EJ, Johnson WW.
Journal: J Pharm Pharmacol (2003): 1251