胰凝乳蛋白酶荧光底物BOC-Val-Pro-Arg-AMC-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
胰凝乳蛋白酶荧光底物BOC-Val-Pro-Arg-AMC价格 1386
产品规格

5 mg

产品货号

胰凝乳蛋白酶荧光底物BOC-Val-Pro-Arg-AMC

产品参数
Ex (nm) 341 Em (nm) 441
分子量 741.75 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:胰凝乳蛋白酶荧光底物BOC-Val-Pro-Arg-AMC

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:741.75

溶剂:DMSO

激发波长(nm):351

发射波长(nm):430

 

产品介绍

胰凝乳蛋白酶荧光底物BOC-Val-Pro-Arg-AMC是美国AAT Bioquest生产的荧光底物,BOC-Val-Pro-Arg-AMC是α-凝血酶的敏感荧光底物。非荧光基板产生亮蓝色荧光AMC产品,其具有Ex / Em = 351 / 430nm,并且可以使用DAPI滤光器组容易地检测。据报道,BOC-Val-Pro-Arg-AMC的α-凝血酶和α-凝血酶卟啉葡萄糖苷复合物的Km值分别为21和25μM。 BOC-Val-ProArg-AMC的α-凝血酶和α-凝血酶葡萄糖凝固酶复合物的Kcat值分别为105和89s-1。该底物也已用于MRSA(耐甲氧西林金黄色葡萄球菌)的快速检测。金畔生物是AAT Bioquest的中国代理商,为您提供优质的胰凝乳蛋白酶荧光底物BOC-Val-Pro-Arg-AMC。 

点击查看光谱

 

参考文献

A fluorescence lifetime-based assay for protease inhibitor profiling on human kallikrein 7
Authors: Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U.
Journal: J Biomol Screen (2009): 1

A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease
Authors: Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP.
Journal: Anal Biochem (2009): 195

A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates
Authors: Nakata H, Ohtsuki T, Sisido M.
Journal: Anal Biochem (2009): 121

Dual-function probe to detect protease activity for fluorescence measurement and 19F MRI
Authors: Mizukami S, Takikawa R, Sugihara F, Shirakawa M, Kikuchi K.
Journal: Angew Chem Int Ed Engl (2009): 3641

Fluorescence resonance energy transfer-based assay for characterization of hepatitis C virus NS3-4A protease activity in live cells
Authors: Sabariegos R, Picazo F, Domingo B, Franco S, Martinez MA, Llopis J.
Journal: Antimicrob Agents Chemother (2009): 728

Improved detection of ovarian cancer metastases by intraoperative quantitative fluorescence protease imaging in a pre-clinical model
Authors: Sheth RA, Upadhyay R, Stangenberg L, Sheth R, Weissleder R, Mahmood U.
Journal: Gynecol Oncol (2009): 616

Label-free, homogeneous, and fluorescence “turn-on” detection of protease using conjugated polyelectrolytes
Authors: An L, Liu L, Wang S.
Journal: Biomacromolecules (2009): 454

Real-time detection of caspase-3-like protease activation in vivo using fluorescence resonance energy transfer during plant programmed cell death induced by ultraviolet C overexposure
Authors: Zhang L, Xu Q, Xing D, Gao C, Xiong H.
Journal: Plant Physiol (2009): 1773

Real-time monitoring of human enterovirus (HEV)-infected cells and anti-HEV 3C protease potency by fluorescence resonance energy transfer
Authors: Tsai MT, Cheng YH, Liu YN, Liao NC, Lu WW, Kung SH.
Journal: Antimicrob Agents Chemother (2009): 748

[Prokaryotic expression and Identification of dual-fluorescence fusion proteins of small ubiquitin-like modifier and sentrin-specific protease]
Authors: Wang X, Guo J, Wang J, Li S, Sun L, Lu J.
Journal: Sheng Wu Gong Cheng Xue Bao (2009): 701

 

相关产品

产品名称 货号
荧光底物BOC-Val-Pro-Arg-Aminoluciferin Cat#13002

Biotin-Val-Ala-Asp(Ome)-fluoromethylketone03FK0140-CF


Biotin-Val-Ala-Asp(Ome)-fluoromethylketone

简要描述:Biotin-Val-Ala-Asp(Ome)-fluoromethylketone,产品型号:03FK0140-CF。
同义词:生物素-Val-Ala-Asp(OMe)-FMK;生物素-VAD(OMe)-FMK
分子式:C30H49FN6O8S

详细介绍

产品咨询

品牌 其他品牌 供货周期 一个月
应用领域 医疗卫生,环保,化工,生物产业,综合

Biotin-Val-Ala-Asp(Ome)-fluoromethylketone,产品型号:90350-38-2

Biotin-Val-Ala-Asp(Ome)-fluoromethylketone,描述:


 生物素-Val-Ala-Asp(Ome)-氟甲基酮是 Z-VAD(OMe)-FMK 的生物素化衍生物,可用于鉴定细胞裂解物中的活性半胱天冬酶。

使用声明

除非另有说明,否则 MP Biomedical 的产品仅供研究或进一步制造使用,不直接供人类使用。如需更多信息,请联系我们的客户服务部门。

关键应用

Caspase 抑制剂/探针

上海金畔生物科技有限公司

  1. 国内试剂耗材经销代理。

  2. 国外试剂的订购。可提供欧美实验室品牌的采购方案。

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  4. 进出口货物代理服务。

  5. 公司代理众多有名生命科学领域的研究试剂、仪器和实验室消耗品品牌

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发光底物BOC-Val-Pro-Arg-氨基荧光素-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
发光底物BOC-Val-Pro-Arg-氨基荧光素价格 7092
产品规格

1 mg

产品货号

发光底物BOC-Val-Pro-Arg-氨基荧光素

产品参数
Ex (nm) 362 Em (nm) 499
分子量 845.91 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:BOC-Val-Pro-Arg-氨基荧光素

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:845.91

溶剂:DMSO

 

产品介绍

        BOC-Val-Pro-Arg-氨基荧光素是美国AAT Bioquest生产的荧光底物,BOC-Val-Pro-Arg-氨基荧光素是α-凝血酶的敏感发光底物。不发光底物产生明亮的发光产物氨基荧光素,其可以用荧光素酶容易地检测。该底物也已用于MRSA(耐甲氧西林金黄色葡萄球菌)的快速检测。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的BOC-Val-Pro-Arg-氨基荧光素。 

 

试剂应用文献

Endotoxin assay by bioluminescence using mutant firefly luciferase
Authors: Kenichi Noda, Hitoshi Goto, Yuji Murakami, Abo Bakr F Ahmed, Akio Kuroda
Journal: Analytical biochemistry (2010): 152–155

 

参考文献

Identification of mutant firefly luciferases that efficiently utilize aminoluciferins
Authors: Harwood KR, Mofford DM, Reddy GR, Miller SC.
Journal: Chem Biol (2011): 1649
 
In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation
Authors: Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.
Journal: Apoptosis (2011): 198
 
Aminoluciferins as functional bioluminogenic substrates of firefly luciferase
Authors: Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.
Journal: Chem Asian J (2011): 1800
 
Noninvasive molecular imaging of apoptosis in vivo using a modified firefly luciferase substrate, Z-DEVD-aminoluciferin
Authors: Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.
Journal: Cell Death Differ (2010): 1003
 
Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase
Authors: Reddy, G. R.; Thompson, W. C.; Miller, S. C.
Journal: J Am Chem Soc (2010): 13586-7
 
Cell-based bioluminescent assays for all three proteasome activities in a homogeneous format
Authors: Moravec RA, O’Brien MA, Daily WJ, Scurria MA, Bernad L, Riss TL.
Journal: Anal Biochem (2009): 294
 
In vivo bioluminescence imaging of furin activity in breast cancer cells using bioluminogenic substrates
Authors: Dragulescu-Andrasi A, Liang G, Rao J.
Journal: Bioconjug Chem (2009): 1660
 
Homogeneous, bioluminescent proteasome assays
Authors: O’Brien MA, Moravec RA, Riss TL, Bulleit RF.
Journal: Methods Mol Biol (2008): 163
 
N-Alkylated 6′-aminoluciferins are bioluminescent substrates for Ultra-Glo and QuantiLum luciferase: new potential scaffolds for bioluminescent assays
Authors: Woodroofe CC, Shultz JW, Wood MG, Osterman J, Cali JJ, Daily WJ, Meisenheimer PL, Klaubert DH.
Journal: Biochemistry (2008): 10383
 
A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers
Authors: Niles AL, Moravec RA, Eric Hesselberth P, Scurria MA, Daily WJ, Riss TL.
Journal: Anal Biochem (2007): 197

 

相关产品

产品名称 货号
胰凝乳蛋白酶荧光底物BOC-Val-Pro-Arg-AMC Cat#13461