锌离子荧光探针Zinquin 乙酯(纯) CAS 181530-09-6-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
锌离子荧光探针Zinquin 乙酯(纯) CAS 181530-09-6价格 1386
产品规格

5 mg

产品货号

锌离子荧光探针Zinquin 乙酯(纯) CAS 181530-09-6

产品参数
Ex (nm) 355 Em (nm) 491
分子量 414.47 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

锌离子荧光探针Zinquin 乙酯是美国AAT Bioquest生产的用于检测锌离子的荧光探针,Zinquin乙酯是亲脂性的,锌敏感的,可透过细胞的荧光探针。它保留在活细胞中,因为乙酯被细胞溶质酯酶切割,产生携带负电荷的Zinquin,阻止其流过质膜。Zinquin荧光探针可以通过在含有5-40μMZinquin乙酯的PBS中与钙和镁(或在培养基中)的培养基中加载到细胞中。通常将细胞与Zinquin乙酯在37℃下孵育15-30分钟。确切的加载浓度,时间和温度取决于实验目的和细胞类型,因此需要通过实验优化。用PBS在培养基中洗涤细胞以除去细胞外剩余的染料。在显微镜下观察细胞或用于共聚焦显微镜,FACS或荧光分光光度法分析。锌是体内第二丰富的过渡金属,它作为催化,结构和调节离子必不可少。锌离子参与体内平衡,免疫反应,氧化应激,细胞凋亡和衰老。已经提出锌作为突触前神经元的常规神经递质和跨越突触后神经元的跨膜信号起作用。异常的锌代谢与许多神经系统疾病有关,包括阿尔茨海默病,帕金森病和癫痫。已证明最适合体内监测锌的技术是荧光成像。检测限:~10 uM。金畔生物是AAT Bioquest的中国代理商,为您提供优质的锌离子荧光探针Zinquin 乙酯。 

 

适用仪器


流式细胞仪  
Ex: 355 nm
Em: 515/30  nm 
实验方案

操作步骤

 1.在DMSO中制备~10mM储备溶液。
 2.装入含有5-40μMZinquin乙酯或PBS(含钙和镁)的培养基中的细胞中。
 3.在37ºC下与Zinquin乙酯一起孵育15-30分钟。确切的加载浓度,时间和温度取决于实验目的和细胞类型,因此需要通过实验优化。
 4.用PBS洗涤以除去细胞外剩余染料,并用PBS或培养基替换。
 5.在显微镜下观察或用流式细胞仪分析。
 
 
参考文献

Zinquin identifies subcellular compartmentalization of zinc in cortical neurons. Relation to the trafficking of zinc and the mitochondrial compartment
Authors: Colvin RA, Laskowski M, Fontaine CP.
Journal: Brain Res (2006): 1

S-Nitroso compounds interfere with zinc probing by Zinquin
Authors: Jansen S, Arning J, Dulcks T, Beyersmann D.
Journal: Anal Biochem (2004): 145

Coordination and fluorescence of the intracellular Zn2+ probe [2-methyl-8-(4-toluenesulfonamido)-6-quinolyloxy]acetic acid (Zinquin A) in ternary Zn2+ complexes
Authors: Hendrickson KM, Geue JP, Wyness O, Lincoln SF, Ward AD.
Journal: J Am Chem Soc (2003): 3889

Fluorescent detection of Zn(2+)-rich vesicles with Zinquin: mechanism of action in lipid environments
Authors: Snitsarev V, Budde T, Stricker TP, Cox JM, Krupa DJ, Geng L, Kay AR.
Journal: Biophys J (2001): 1538

The synthesis and fluorescent properties of analogues of the zinc(II) specific fluorophore zinquin ester
Authors: Kimber MC, Mahadevan IB, Lincoln SF, Ward AD, Tiekink ER.
Journal: J Org Chem (2000): 8204

Changes in distribution of labile zinc in mouse spermatozoa during maturation in the epididymis assessed by the fluorophore Zinquin
Authors: Zalewski PD, Jian X, Soon LL, Breed WG, Seamark RF, Lincoln SF, Ward AD, Sun FZ.
Journal: Reprod Fertil Dev (1996): 1097

Flux of intracellular labile zinc during apoptosis (gene-directed cell death) revealed by a specific chemical probe, Zinquin
Authors: Zalewski PD, Forbes IJ, Seamark RF, Borlinghaus R, Betts WH, Lincoln SF, Ward AD.
Journal: Chem Biol (1994): 153

Measurement of zinc in hepatocytes by using a fluorescent probe, zinquin: relationship to metallothionein and intracellular zinc
Authors: Coyle P, Zalewski PD, Philcox JC, Forbes IJ, Ward AD, Lincoln SF, Mahadevan I, Rofe AM.
Journal: Biochem J (1994): 781

锌离子荧光探针Zinquin CAS 151606-29-0-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
锌离子荧光探针Zinquin CAS 151606-29-0价格 1386
产品规格

5 mg

产品货号

锌离子荧光探针Zinquin  CAS 151606-29-0

产品参数
Ex (nm) 355 Em (nm) 491
分子量 386.42 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:锌离子荧光探针Zinquin 

CAS:151606-29-0

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:386.42

外观:淡黄色固体

溶剂:DMSO

激发波长(nm):355

发射波长(nm):491

 

产品介绍

锌离子荧光探针Zinquin 是美国AAT Bioquest生产的用于检测锌离子的荧光探针。Zinquin是一种锌敏感的,细胞不可渗透的荧光探针。锌是人体中第二丰富的过渡金属,它是催化,结构和调节离子必不可少的元素。锌离子参与体内平衡,免疫反应,氧化应激,细胞凋亡和衰老。已经提出锌起突触前神经元的常规神经递质的作用,并作为横穿突触后神经元的跨膜信号。锌代谢异常与许多神经系统疾病有关,包括阿尔茨海默氏病,帕金森氏病和癫痫病。已经证明用于体内锌监测的最合适技术是荧光成像。金畔生物是AAT Bioquest的中国代理商,为您提供优质的锌离子荧光探针Zinquin 。 

点击查看光谱

 

参考文献

Zinquin identifies subcellular compartmentalization of zinc in cortical neurons. Relation to the trafficking of zinc and the mitochondrial compartment
Authors: Colvin RA, Laskowski M, Fontaine CP.
Journal: Brain Res (2006): 1

S-Nitroso compounds interfere with zinc probing by Zinquin
Authors: Jansen S, Arning J, Dulcks T, Beyersmann D.
Journal: Anal Biochem (2004): 145

Coordination and fluorescence of the intracellular Zn2+ probe [2-methyl-8-(4-toluenesulfonamido)-6-quinolyloxy]acetic acid (Zinquin A) in ternary Zn2+ complexes
Authors: Hendrickson KM, Geue JP, Wyness O, Lincoln SF, Ward AD.
Journal: J Am Chem Soc (2003): 3889

Fluorescent detection of Zn(2+)-rich vesicles with Zinquin: mechanism of action in lipid environments
Authors: Snitsarev V, Budde T, Stricker TP, Cox JM, Krupa DJ, Geng L, Kay AR.
Journal: Biophys J (2001): 1538

The synthesis and fluorescent properties of analogues of the zinc(II) specific fluorophore zinquin ester
Authors: Kimber MC, Mahadevan IB, Lincoln SF, Ward AD, Tiekink ER.
Journal: J Org Chem (2000): 8204

Changes in distribution of labile zinc in mouse spermatozoa during maturation in the epididymis assessed by the fluorophore Zinquin
Authors: Zalewski PD, Jian X, Soon LL, Breed WG, Seamark RF, Lincoln SF, Ward AD, Sun FZ.
Journal: Reprod Fertil Dev (1996): 1097

Flux of intracellular labile zinc during apoptosis (gene-directed cell death) revealed by a specific chemical probe, Zinquin
Authors: Zalewski PD, Forbes IJ, Seamark RF, Borlinghaus R, Betts WH, Lincoln SF, Ward AD.
Journal: Chem Biol (1994): 153

Measurement of zinc in hepatocytes by using a fluorescent probe, zinquin: relationship to metallothionein and intracellular zinc
Authors: Coyle P, Zalewski PD, Philcox JC, Forbes IJ, Ward AD, Lincoln SF, Mahadevan I, Rofe AM.
Journal: Biochem J (1994): 781

锌离子荧光探针Zinquin AM-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
锌离子荧光探针Zinquin AM价格 2823
产品规格

1 mg

产品货号

锌离子荧光探针Zinquin AM

产品参数
Ex (nm) 355 Em (nm) 491
分子量 458.48 溶剂 DMSO
存储条件 在零下15度以下保存, 避免光照
产品概述

锌离子荧光探针Zinquin AM是美国AAT Bioquest生产的用于锌离子检测的荧光探针,Zinquin AM酯是一种亲脂的,锌敏感的,可透过细胞的荧光探针。它被保留在活细胞中,因为AM酯被细胞溶质酯酶切割,产生携带负电荷的Zinquin,防止其流过质膜。Zinquin荧光探针可以通过在含有5-40μMZinquin乙酯的PBS中与钙和镁(或在培养基中)的培养基中加载到细胞中。通常将细胞与Zinquin乙酯在37℃下孵育15-30分钟。确切的加载浓度,时间和温度取决于实验目的和细胞类型,因此需要通过实验优化。用PBS在培养基中洗涤细胞以除去细胞外剩余的染料。在显微镜下观察细胞或用于共聚焦显微镜,FACS或荧光分光光度法分析。锌是体内第二丰富的过渡金属,它作为催化,结构和调节离子必不可少。锌离子参与体内平衡,免疫反应,氧化应激,细胞凋亡和衰老。已经提出锌作为突触前神经元的常规神经递质和跨越突触后神经元的跨膜信号起作用。异常的锌代谢与许多神经系统疾病有关,包括阿尔茨海默病,帕金森病和癫痫。已证明适合体内监测锌的技术是荧光成像。免疫反应,氧化应激,细胞凋亡和衰老。已经提出锌作为突触前神经元的常规神经递质和跨越突触后神经元的跨膜信号起作用。异常的锌代谢与许多神经系统疾病有关,包括阿尔茨海默病,帕金森病和癫痫。已证明适合体内监测锌的技术是荧光成像。免疫反应,氧化应激,细胞凋亡和衰老。已经提出锌作为突触前神经元的常规神经递质和跨越突触后神经元的跨膜信号起作用。异常的锌代谢与许多神经系统疾病有关,包括阿尔茨海默病,帕金森病和癫痫。已证明适合体内监测锌的技术是荧光成像。金畔生物是AAT Bioquest的中国代理商,为您提供优质的锌离子荧光探针Zinquin AM。 

点击查看实验方案

点击查看光谱

 

底读模式

实验方案

操作步骤

1.准备HHBS缓冲液
 

2.在高质量无水DMSO中制备2 mM至5 mM Zinquin AM * UltraPure级*原液。
2.1使用的Zinquin AM * UltraPure等级*的量:1毫克
2.2所需浓度:2 mM
2.3在合适的容器中,将1mg Zinquin AM * UltraPure级*与1090.56μL无水DMSO混合。
 

3.使用10μMZinquinAM * UltraPure等级* 2在HHBS中制备2X工作溶液
3.1Zinquin AM * UltraPure等级的终井内浓度*:5μM
3.2在合适的容器中混合16μL的Zinquin AM * UltraPure等级*。然后,添加HHBS或您选择的缓冲液,直到体积为3.2毫升。
注意:对于大多数细胞系,我们建议Z​​inquin AM * UltraPure等级的终浓度为4至5μM。
 

4.将100μL染料工作溶液加入已经含有100μL培养基的所需孔中。
4.1该步骤将染料工作溶液从2X稀释至1X,并将每种组分的终浓度调节至以下:5μMZinquinAM * UltraPure等级*
 

5.孵育染料
5.1将染料加载板在细胞培养箱中孵育30-60分钟。
5.2将染料加载板在室温下孵育30分钟。
 

6.准备HHBS缓冲液(或您选择的缓冲液)。
6.1在合适的容器中加入HHBS或您选择的缓冲液,直至体积为4 mL。
 

7.用HHBS缓冲液或您选择的缓冲液替换染料工作溶液,使用1.0 mM Probenecid。
7.1首先,从所需孔中除去200μL染料工作溶液和培养基。
7.2在相同的孔中加入200μL含有1.0mM丙磺舒的HHBS(或您选择的缓冲液)。
 

8.运行实验
8.1为您的样品添加所需的处理。
8.2以Ex / Em = 360/480 nm运行实验。

 

参考文献

Zinquin identifies subcellular compartmentalization of zinc in cortical neurons. Relation to the trafficking of zinc and the mitochondrial compartment
Authors: Colvin RA, Laskowski M, Fontaine CP.
Journal: Brain Res (2006): 1

S-Nitroso compounds interfere with zinc probing by Zinquin
Authors: Jansen S, Arning J, Dulcks T, Beyersmann D.
Journal: Anal Biochem (2004): 145

Coordination and fluorescence of the intracellular Zn2+ probe [2-methyl-8-(4-toluenesulfonamido)-6-quinolyloxy]acetic acid (Zinquin A) in ternary Zn2+ complexes
Authors: Hendrickson KM, Geue JP, Wyness O, Lincoln SF, Ward AD.
Journal: J Am Chem Soc (2003): 3889

Fluorescent detection of Zn(2+)-rich vesicles with Zinquin: mechanism of action in lipid environments
Authors: Snitsarev V, Budde T, Stricker TP, Cox JM, Krupa DJ, Geng L, Kay AR.
Journal: Biophys J (2001): 1538

The synthesis and fluorescent properties of analogues of the zinc(II) specific fluorophore zinquin ester
Authors: Kimber MC, Mahadevan IB, Lincoln SF, Ward AD, Tiekink ER.
Journal: J Org Chem (2000): 8204

Changes in distribution of labile zinc in mouse spermatozoa during maturation in the epididymis assessed by the fluorophore Zinquin
Authors: Zalewski PD, Jian X, Soon LL, Breed WG, Seamark RF, Lincoln SF, Ward AD, Sun FZ.
Journal: Reprod Fertil Dev (1996): 1097

Flux of intracellular labile zinc during apoptosis (gene-directed cell death) revealed by a specific chemical probe, Zinquin
Authors: Zalewski PD, Forbes IJ, Seamark RF, Borlinghaus R, Betts WH, Lincoln SF, Ward AD.
Journal: Chem Biol (1994): 153

Measurement of zinc in hepatocytes by using a fluorescent probe, zinquin: relationship to metallothionein and intracellular zinc
Authors: Coyle P, Zalewski PD, Philcox JC, Forbes IJ, Ward AD, Lincoln SF, Mahadevan I, Rofe AM.
Journal: Biochem J (1994): 781